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1* ''WesternAnimation/BarbieAsTheIslandPrincess'': Ro's elephant friend Tika has [[CartoonyEyes large, glassy eyes]] that look more like human eyes Photoshopped onto an elephant's face, and are also ''much'' closer together than a real elephant's eyes would be.
2** Also, when she is in danger of drowning, Tika holds her head above the water. Real elephants use their trunks like snorkels to breathe when swimming.
3* ''WesternAnimation/BarbieAsThePrincessAndThePauper'': Erika's cat Wolfie is described as a calico, but he's [[AnimalGenderBender male]]. Calico cats are almost exclusively female, and in the rare cases males are born, they are sterile due to a genetic disorder.
4* ''WesternAnimation/BeeMovie'':
5** At the beginning, it's mentioned that bees shouldn't, according to the "laws of aviation", be able to fly. Actually, the laws of aviation only prove that a bee and a plane can't fly the same way; it's been known for decades how bees fly.
6** Some of the worker bees are male. In reality, only female bees are workers.
7** Several inaccuracies regarding stingers: firstly, the male bees are portrayed with them (in real life, only female bees have stingers), secondly when Adam stings a man, he's saved from death by being given a prosthetic stinger which wouldn't work in reality, and thirdly when Barry mentions he's going to "drain the stinger" this implies he's [[GoToTheEuphemism referring to using the bathroom]]... even though bees don't poop out their stingers (and don't need to drain their venom either).
8** The plants start dying from not being pollinated. Lack of pollination doesn't kill plants; it just prevents more plants from being made. Also, bees aren't the only things that pollinate, and plants can't be pollinated from a different species of plant.
9** A singer named Music/{{Sting}} gets arrested for his name being a "bee thing", even though bees are far from the only animal that stings.
10* ''WesternAnimation/ABugsLife'': This comes from there being male worker ants -- in real life, all worker ants are females, and at the very least, the male ants should have wings. In the video game adaptation, there is even a scene where Flik the male ant says "And they say male ants can't fly!".
11* ''WesternAnimation/Beowulf2007'': Going past all of the usual dragon examples that would apply to the beast (like wingspan), how does a heart that can fit in a man's fist pump blood through the body of a [[AttackOfThe50FootWhatever seventy-foot-long]] [[IfItSwimsItFlies flying and swimming]] reptile? Never mind that a heart in the neck protected by tracing paper is a bad idea anyway. Blocking the trachea and being easily ripped out are not desirable traits in a heart. However this is a human-demon hybrid, so there is an element of the supernatural at work which can circumvent the biological impossibilities.
12* ''WesternAnimation/{{Coco}}'': Despite being skeletons, the characters still have lips that cover their teeth. An obvious tradeoff to allow them to emote.
13* ''WesternAnimation/{{Dumbo}}'' features a few examples of circus animals doing stuff they don't do (ostriches putting their heads in the sand, for example), but probably the most egregious is during "Baby Mine" when we see hippos sleeping underwater. [[AccidentallyCorrectWriting This is actually possible]], but only due to a reflex that lets hippos surface every couple of minutes and breathe air while remaining asleep. They don't stay submerged the whole time and breathe water as portrayed in the film - like any other mammal, they would drown.
14* ''WesternAnimation/TheEmperorsNewGroove'': When Pacha tries to give Kuzco CPR, his long llama tongue pops out and flops on the ground. In reality, llama tongues are ''incredibly'' short; they can only reach half an inch out of their mouth.
15* ''WesternAnimation/FindingNemo'':
16** Barracudas don't usually attack eggs. They prefer live prey. That said, it's possible the barracuda was aiming for Coral and destroyed the eggs by accident.
17** Also, clownfish don't keep their eggs in caves for the very reason that happened in the movie. To keep their eggs safe from anything that would eat the eggs, they lay them on a flat part of the sea anemone where egg-eaters can't get them. But you need some reason to get the plot going...
18** The interior of the whale's throat is too large. While blue whales have a gigantic '''''mouth''''', their throat is tiny and unable to swallow anything larger than a beach ball (though that is still enough to swallow Marlin and Dory). They also have respiratory and digestive traits completely separated, you know, so that they don't unintentionally [[spoiler:sneeze fish out of their blowhole]]. [[HollywoodDarkness And where is the light inside its mouth coming from?]] Also, whales don't have uvulas.
19** Chuckles, the gift fish who was killed by Darla, looks like a goldfish. Which live in freshwater. The other Tank Gang fish are saltwater fish.
20** None of the sea turtles seem to worry about having to breathe. They also don't travel in flocks, [[RuleOfCool but this was intentional]].
21** Sea turtles don't live anywhere near 150 years; that honor belongs to tortoises. Their expected lifespan is still quite impressive at 80 years.
22** Clownfish do live in anemones, but they also live in harems dominated by one male and one female, with a lot of non-productive males in the rest. When the dominant female dies, the dominant male undergoes a GenderBender and becomes the new dominant female. Clownfish will also reproduce with their relatives in times of emergency. This particular tidbit has raised eyebrows at the choice of clownfish for the film.
23** Flapjack octopi have their mouths underneath their heads, not right below the eyes like a human face.
24** Interestingly, a featurette on DVD addresses the whole Artistic License issue. An animator relates a story of one of their consultants talking about the biological inaccuracies in their final fish designs. The animator replied, sheepishly, "Well... in real life they don't ''talk'' either, so..."
25* In ''WesternAnimation/{{How to Train Your Dragon|2010}}'', Hiccup is out of bed and walking on an artificial leg just a few days after losing his foot. In RealLife, amputees take weeks or even ''months'' to heal enough to even be fitted for a prosthetic, let alone be using it.
26* ''WesternAnimation/IceAge'':
27** There is a creature which is identified as an aardvark, but though it has the ears of an aardvark, it has the bushy tail of a giant anteater, and its snout is weirdly elongated to be reminiscent of an anteater (not an aardvark), but that animal would have a tiny mouth at the tip of its snout, whereas the cartoon critter has its mouth at the base.
28** Scrat the proto-squirrel has huge saber-like canine teeth. Being rodents, squirrels -- even prehistoric ones -- don't have canines at all. Although the authors have said in an interview that it was PlayedForLaughs. Later crosses into AccidentallyCorrectWriting since [[http://www.npr.org/blogs/thetwo-way/2011/11/03/141997834/scientists-unveil-fossil-of-saber-toothed-squirrel-that-lived-among-dinos a later discovered prehistoric mammal was indeed squirrel-like]], [[ScienceMarchesOn and did indeed have fangs]]. It was not a rodent though, and lived in the ''Mesozoic'', not in the Cenozoic, much less the last ice age.
29** Sid and the other sloths walk perpetually upright and flat on their feet (sloths walk on all fours and on the sides of their feet, not unlike an anteater), and have heads shaped like a ''hammerhead''. They also have noses more like a dog's, though it's most likely ground sloths had pig-like noses since both 3-toed and 2-toed sloths have them.
30* ''WesternAnimation/JonahAVeggieTalesMovie'': The opening features a mother porcupine who launches her quills at the campers' bus to pop their tires. Though real porcupines have loosely attached quills that can come off with enough momentum, intentionally shooting them as projectiles is pure fiction.
31* ''WesternAnimation/TheLegendOfTheTitanic'':
32** The dolphins jump as high as the deck of Titanic and manage to float in the air for a short amount of time by ''flapping their flippers''.
33** The unrealistically large octopus which has a dog's nose, and has to take a breath before it goes underwater.
34* ''WesternAnimation/{{Leo}}'': Leo combines features from a bunch of different lizards, like iguanas, geckos and chameleons, and is supposedly a tuatara (a lone surviving species of prehistoric reptile that are not actually lizards).
35** Squirtle is shown to be able to remove his shell, an impossibility for real terrapins as the top of their shells are actually fused to their spines.
36** InUniverse, PlayedForLaughs example: when one of the kids asks Squirtle where babies come from, he gives them a rundown on ''turtle'' reproduction instead of human. As for why a terrestrial box turtle in a fifth grade classroom would know the mating habits of sea turtles, best not to look too far into it.
37* ''WesternAnimation/{{Madagascar}}'': Fossa prefer to live on their own, not in a pack.
38* ''WesternAnimation/TheNightmareBeforeChristmas'': If Finkelstein, especially at his age, removed half his brain like he does to create his "wife", he would lose motion on one side of his body (and since he's already a GeniusCripple, that would mean he has only one working limb) and would, depending on which side he removed, develop speech problems. At the same time, his wife would be equally crippled. Not to mention hemispherectomies are very delicate procedures; you don't break apart a brain like a loaf of bread. It could easily be a case of BizarreAlienBiology.
39* ''Film/OsmosisJones'': If Thrax were actually a virus, he would be about as tall as Jones's ankle.
40* ''WesternAnimation/{{Pinocchio}}'':
41** Jiminy Cricket bears very little, if any, resemblance to an actual cricket. He was originally depicted as an actual (that is, less anthropomorphized) cricket with toothed legs and waving antennae. But Walt wanted something more appealing, so he assigned animator Creator/WardKimball to redesign the cricket. Kimball whittled away at any and all cricket-like appendages until Jiminy became, in Kimball's words, "a little man with an egg head and no ears. And the only thing that makes him a cricket is because we call him one."
42** Monstro the whale resembles a sperm whale, but has a head that's much too wide and teeth on both jaws, when real sperm whale have laterally-flattened heads and only have teeth on their very thin lower jaw. He also has belly lining, which is a trait of baleen whales, and is seen sleeping at the bottom of the ocean. Whales don't sleep that way; they would drown otherwise. Although it's eventually revealed he's just pretending to sleep so he can ambush passing schools of fish.
43* ''WesternAnimation/ThePrincessAndTheFrog'': The alligators that attack Tiana and Naveen are depicted with interlocking teeth like crocodiles. It gets jarring in that Louis has his lower teeth hidden when he closes his mouth, like a real alligator.
44* In Creator/RankinBassProductions' ''[[WesternAnimation/SantaClausIsCominToTown The Easter Bunny Is Comin' to Town]]'', one music segment has the chickens tell a story [[BigLippedAlligatorMoment in a song]] that makes fun of the riddle of "chicken or the egg": They explain that "the chicken came first" by retelling Literature/TheBible story of [[TheGreatFlood Noah's Ark]], and comparing the riddle to who came first: "[[NurseryRhyme the pussycat or the fiddle]]", "the [[http://en.wikipedia.org/wiki/Fountain_of_Youth Fountain]] or [[https://en.wikipedia.org/wiki/Juan_Ponce_de_León Ponce de León]]", and "[[BreadEggsMilkSquick the cow or]] [[https://en.wikipedia.org/wiki/Catherine_O%27Leary Mrs. O'Leary]]". ScienceMarchesOn: Scientists now think the dinoid oviducts had to evolve to produce the proper egg before the chicken('s ancestor) could be hatched. Technically, the chicken did evolve first.
45* ''WesternAnimation/{{Ratatouille}}'':
46** Rémy gags when he catches a whiff of the soup Linguini has clumsily doctored. Rats are physically incapable of gagging. RuleOfFunny applies, of course.
47** Onions and scallions can cause anemia when ingested by rats so despite Rémy taste-testing many of the dishes he prepared, license is taken that he never ingests enough to trigger a toxic reaction.
48* ''WesternAnimation/TheRescuers'': There seems to be some inconsistency as to whether Brutus and Nero are alligators or crocodiles. If they are alligators (as the concept art for the movie implies), then their teeth are incorrect, being shown protruding instead of covered by the upper jaw, plus they are colored green like crocs rather than gray like real gators.
49* ''WesternAnimation/{{Sahara}}'':
50** Each snake sports a unique scale color and patterning unlike anything exhibited by real snakes. In Eva's case, her pattern consists of a row of ornate flowers imitating the style of Mehndi body art, also known as henna[[https://en.wikipedia.org/wiki/Mehndi]].
51** The Dusty snakes are shown eating watermelon and chewing it before swallowing. Real-life snakes are obligate carnivores (they won't eat anything other than meat), and they swallow their prey whole instead of chewing on it.
52** Snakes in real life exhibit a wide variety of colors and patterns meant to act as camouflage or signal that they are venomous. The patterns displayed by the snakes in the film, however, are unlike anything found on actual serpents. The colors and patterns seen in the film are instead designed to give each character a unique appearance and reflect some aspect of their personality. This is rather apparent in Saladin's case as his hood markings resemble a skull.
53* On ''WesternAnimation/StrawberryShortcake'', the Purple Pieman apparently literally never dreamed throughout his life up until ''The Sweet Dreams Movie'' — that's why he wants to "steal a little dream of yours." That's biologically impossible — people who don't dream go insane and eventually die.
54* ''WesternAnimation/{{Tarzan}}'':
55** Tarzan's often able to win over others' sympathy through his eyes, including the gorillas. In reality, gorillas do not like direct eye contact, perceiving it as a challenge. Locking eyes with one of them is demanding a fight.
56** A human walking on his knuckles as Tarzan does would be extremely painful and cause severe damage to the bones in the hands. Gorillas get away with this due to thicker knuckle bones and arms longer than their legs, unlike humans. As he's spent most of his life walking on all fours, Tarzan's pretty fortunate that his back isn't wrecked as well.
57** Albeit with great difficulty, Tarzan does manage to physically hold Kerchak back to stop him from attacking Jane. Though it is difficult to gauge their strength with any precision, a silverback male gorilla usually tips the scales at about 400 pounds and is many times strong than any human. A human attempting to take on an angry silverback in real life would literally be crushed in a heartbeat.
58* In ''WesternAnimation/TurningRed'', Mei's giant red panda form is shown to have a prehensile tail unlike real red pandas.

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