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* ''VisualNovel/DanganRonpa'' has Monokuma with his "Upupupupu..." and [[spoiler:[[SerialKiller Genocider Shou]]/[[DubNameChange Genocide Jack's]]]] "GYAHAHAHAHAHAHA!" which she tends to introduce herself with. The English translation changed Monokuma's laugh to "Puhuhuhu..." ("Nyek Nyek Nyek Nyek!" in TheAnimeOfTheGame) and [[spoiler:Jack's]] to "Kyeehahahaha!"

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* ''VisualNovel/DanganRonpa'' ''{{Franchise/Danganronpa}}'':
** ''VisualNovel/DanganronpaTriggerHappyHavoc''
has Monokuma with his "Upupupupu..." and [[spoiler:[[SerialKiller Genocider Shou]]/[[DubNameChange Genocide Jack's]]]] "GYAHAHAHAHAHAHA!" which she tends to introduce herself with. The English translation changed Monokuma's laugh to "Puhuhuhu..." ("Nyek Nyek Nyek Nyek!" in TheAnimeOfTheGame) and [[spoiler:Jack's]] to "Kyeehahahaha!"

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* ComicBook/WonderWoman villain and misogynistic slave owner Uvo's "Ho-hoe!" laugh.

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* ''VideoGame/CookieRun'': Herb Cookie's "Hoo-hoo!"

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* ''WesternAnimation/ReadyJetGo'': Jet Propulsion's signature "HA-HA-HA-ha-HA!"

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* Creator/ElainePaige presents a regular musical theatre show for Creator/{{BBC}} Radio Two. Her signature laugh is extremely distinctive indeed and unkind people have speculated that [[AnnoyingLaugh this may be the reason why]] Creator/AndrewLloydWebber divorced her.

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** The elderly turtle-looking Waterfall shopkeeper continually lets out a bossy "Wah ha ha!" It even features if you enter his name ([[spoiler:Gerson]]) on the [[HelloInsertNameHere starting name entry screen]].

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* ''VideoGame/Dota2'' has Sniper and his distinctive "HO HO! HA HA!" It's far from his only laugh, but players are very well acquainted with it since he uses that particular laugh every other time he uses an ability. [[Creator/ValveSoftware Valve]] even got in on the act with one of his cosmetic items, an oversized reel of carnival tickets [[AscendedMeme with "HO HO HA HA" printed on every single one]].

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* Ai from ''VideoGame/{{Yakuza 0}}'' has a very distinct "Ehehe."


* Disney/MickeyMouse's high-pitched giggle from when Creator/WaltDisney was voicing him. Creator/WayneAllwine's short "Ha-ha!" from more recent times is almost as memorable.

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* Disney/MickeyMouse's WesternAnimation/MickeyMouse's high-pitched giggle from when Creator/WaltDisney was voicing him. Creator/WayneAllwine's short "Ha-ha!" from more recent times is almost as memorable.


* Several ''WesternAnimation/{{Batman}}'' villains:

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* Several ''WesternAnimation/{{Batman}}'' ''WesternAnimation/{{The Batman}}'' villains:

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* The concept album ''Märchen'' by Music/SoundHorizon has the VillainProtagonist's sidekick Elise, whose speaking voice actress Creator/SakiFujita gives her a very distinctive EvilLaugh.

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* [[https://www.youtube.com/watch?v=n3J2jDQ9cOEsnyc The Pillsbury Doughboy's]] giggle in response to having his tummy poked.


* Music/WolfgangAmadeusMozart's AnnoyingLaugh in ''Music/{{Amadeus}}'' announces his presence whenever he's in the area.

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* Music/WolfgangAmadeusMozart's AnnoyingLaugh in ''Music/{{Amadeus}}'' ''Film/{{Amadeus}}'' announces his presence whenever he's in the area.


* Disney/MickeyMouse's high-pitched giggle from when Creator/WaltDisney was voicing him. Wayne Allwine's short "Ha-ha!" from more recent times is almost as memorable.

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* Disney/MickeyMouse's high-pitched giggle from when Creator/WaltDisney was voicing him. Wayne Allwine's Creator/WayneAllwine's short "Ha-ha!" from more recent times is almost as memorable.

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* Sublieutenant Phillips' 'silly-ass' laugh in ''Radio/TheNavyLark'' (usually at his own jokes). Most of the other characters find it irritating, especially Captain Povey.

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