* AssholeVictim: [[spoiler: Madam M]]
* DangerousForbiddenTechnique - The assassins have many ways of killing people, but their signature move involves ''pulling out the target's spinal cord''. At first, it looks like the move takes a bit of time. Later, the assassins are doing it in the middle of a firefight.
** That's nothing, in the climax [[spoiler:Charlene kills the BigBad by throwing him and performing the move while he's flying through the air in bullet-time]].
* DeadlyGraduation - After years of assassin training, Madam M has half of the trainees take out the other half. Then the next day, the survivors compete to the death until only one is standing. [[spoiler:Madam M ultimately changes her mind and spares the last surviving three.]]
* FemmeFatale - This film is essentially one part Femme Fatale MurderInc, one part TrainingFromHell
* ForcedToWatch - Charlene is forced to watch [[spoiler:the Yakuza man kill her best friend]].
* GratuitousRape - The surviving assassins are subjected to this by the men working for Madam M.
* HoneyTrap - How the assassins get close to their marks.
* ItsPersonal - Jack lost his original partners thanks to Madam M and spends every available moment of his detective career looking into her MurderInc, earning him the ridicule of colleagues, who don't believe such a thing exists.
* MakingLoveInAllTheWrongPlaces - Charlene and Jack end up having sex on a beach at night. Jack even admits he doesn't normally sleep with girls so soon after meeting them.
* MurderInc - Madam M's organization is supposedly a notoriously effective one of these.
* ProfessionalKiller - What the girls in this film are being trained to become.
* SlippingAMickey - Madam M does this to her surviving assassins. Aforementioned GratuitousRape soon follows.
* TrainingFromHell - During their years of training, nearly all of the girls will be brutally killed.
* TheVamp - One of the skills the assassins are specifically trained to utilize.
* WineIsClassy - During a dinner, the surviving girls are tested on their ability to discern wine. Two glasses turn out to be full of expensive vintages, while one is common table wine. [[spoiler:The real purpose is to drug the girls, so her mercenaries can gang-rape them.]]
* WouldHurtAChild - Madam M
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