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** [[spoiler:The canon turtles are all just excepted to be brothers with Splinter being the source of their human DNA, making Karai their sister on a genetic level. In Chapter 19, it turns out that back when they were all nine, Donatello was able to have their DNA tested. It revealed that Leo and Mikey are paternal half-brothers (pre-mutation) with two unrelated humans being the source of their human DNA. Raphael is Mikey's third cousin on his human side. Donatello is unrelated to all three of them on all sides.]] [[spoiler:When Karai and Donatello where imprisoned and tortured by the Foot, Donnie confesses to her that his DNA came from Splinter and has been keeping this a secret from the others, making him the only turtle that is Splinter's biological son and Karai's half-sibling.]]
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** [[spoiler:The canon turtles are all just excepted accepted to be brothers with Splinter being the source of their human DNA, making Karai their sister on a genetic level. In Chapter 19, it turns out that back when they were all nine, Donatello was able to have their DNA tested. It revealed that Leo and Mikey are paternal half-brothers (pre-mutation) with two unrelated humans being the source of their human DNA. Raphael is Mikey's third cousin on his human side. Donatello is unrelated to all three of them on all sides.]] [[spoiler:When Karai and Donatello where imprisoned and tortured by the Foot, Donnie confesses to her that his DNA came from Splinter and has been keeping this a secret from the others, making him the only turtle that is Splinter's biological son and Karai's half-sibling.]]
