* AdaptationDisplacement: The TV show is better known to some than the original books; in fact, a new series of paperbacks went into print based on the TV episodes.
* AlternativeCharacterInterpretation:
** Is Ms. Frizzle something like a Willy Wonka who is teaching the class to find a successor to the Magic School Bus?
** Is Ms Frizzle a normal human or not? She's implied to be [[OlderThanTheyLook older]] than her roughly 30-to-mid-40s look, and she is into magic.
** Is the school bus really magic or is it science? Or perhaps [[{{Magitek}} both?]] It's an oddity that a science teacher would be involved with magic. ClarkesThirdLaw comes into effect.
* CrackShip:
** Some of the fandom likes involving Ms. Frizzle in crossover lesbian relationships with Film/MaryPoppins (both are [[Series/DoctorWho Time Lords]], apparently) or Franchise/CarmenSandiego.
** According to Tumblr, Carlos apparently grows up to be the perfect, wonderful scientist who moves into [[Podcast/WelcomeToNightVale Night Vale]] and falls in love with the host, Cecil.
* CrazyAwesome: Ms. Frizzle of course. It's probably her defining character trait.
* EarWorm: The theme song (performed by Music/LittleRichard), and the {{leitmotif}} of the start of each field trip that derives from it.
* EnsembleDarkhorse: [[FountainOfMemes Carlos.]]
* HilariousInHindsight:
** In "Out of This World," aired years before [[ScienceMarchesOn Pluto's demotion]], the class is rehearsing a play about the solar system where they each play a different planet, but Dorothy Ann is late, so they had to start without her planet... ''Pluto''.
** In "The Busasaurus" Carlos says "Mmm-mmm, Website/ThatGuyWithTheGlasses was good!"
** [[Series/MindOfMencia A terrible Hispanic comedian named Carlos...]]
* LauncherOfAThousandShips: Arnold. He's been shipped with ''everyone'' - everyone in his class, Ms. Frizzle, Molly Cule, that guy from the hot cocoa episode (Harry Arm), that girl from the water cycle episode (Tiffany), Janet, [[WesternAnimation/AvatarTheLastAirbender Az]][[CrackShip u]][[CrossoverShip la]]...
* LoveToHate: Janet, Janet, a thousand times over, Janet.
* MemeticMutation:
** "CARLOS!" [[LamePunReaction as a response to]] his [[{{Pun}} terrible puns]].
** Footage from the show seems to be popular for WebAnimation/YouTubePoop (although, given the NostalgiaFilter protection the show has garnered in North America, it's probably {{affectionate|Parody}} more than anything).
** "I knew I should have stayed home today..."
** In Israel, the show was just called ‘The Magic Bus’ (because school buses are not as ubiquitous there). Unfortunately, ‘magic’ (or, more accurately, ‘acts of magic’) sounds a lot like ‘drugs’ in Hebrew (קסמים ''ksamím'' vs. סמים ''samím'', respectively). Cue many jabs from kids who’ve outgrown the series about the bus adventures being a case of MushroomSamba.
** "[[https://www.youtube.com/watch?v=aahPUCyS5Qo Girl that's a booty hole.]]"
* NightmareFuel: While on Pluto, Arnold takes his helmet off and [[SpaceIsCold his head freezes]]. As well as the extremely creepy sound episode.
* PeripheryDemographic: The series has gained a large amount of older fans, many of whom grew up with it. It helps that both the facts (for the most part) are still relevant and the characters are interesting. Older fans have been known to revisit the show because of how much they forgot that they had learned in elementary.
* TheScrappy:
** Janet. To be fair though, this was probably intentional.
** Tim is this to a small extent, mostly for being TheGenericGuy--he's not ''hated'', per se, but he doesn't have as big of a fanbase compared to the other characters (but he does have some fans).
* {{Squick}}: Some of the Fantastic Voyage moments:
** For example, the episode where the kids go inside Janet's nose.
** Or when the class was inside Arnold's large intestine, getting up close and personal with piles of poop.
** Or when the class [[AccidentalInnuendo got laid]] by a chicken.
* TheWoobie:
** Everyone has their moments, but Arnold probably has the most.
** Keesha gets special mention, during her TenMinuteRetirement in "Gets Ants in its Pants".
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