These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
Designated Villain: Hades counts since Frollo, who had once been his friend, had for no reason turned on him, broken his rurus and had Lefou shit on his face.
Let's not forget Konata who along with her friends was murdered and eaten by Bison and Guile in Leet Fighters. The revenge she seeks is completely justified (its just as reasonable as Uma Thurman's character from Kill Bill wanting revenge). Leet Fighters not only treats her character as a villain but plays her up entirely for laughs with her character getting killed every time she almost gets revenge. But that kind of dark humor is to be expected from chincherrinas.
Dude, Not Funny!: Marco teasing Haruhi with a flying shoe might count as this, given how in the previous episode He almost killed her in an attack with it, and did end up killing her friend, Maussan.
Genius Bonus: In Frollo Gets Flashed by a Gothic Lolita, it's stated that the Nazis are attacking from the right and the Communists from the left. This could just be a coincidence. Or it could be a joke based on their positions on the political spectrum.
Growing the Beard: Around the time of Frollo Gets AIDS, the series starts getting a sense of continuity and becomes less of just a Youtube Poop. The creator of the series doesn't even consider the show to be Youtube Poop anymore.
The Bros Pose is slowly reaching meme status, as chincherrinas encourages anyone to make their own poses. You can view the fan-made Bros Pose gallery here, but be warned; a few of them are not well-done.
That One Boss: Ronald McDonald effortlessly dodges Frollo's bullets, knocks his gun right out of his hand...Ayumu is weak against clowns...and to top it all off, Gameshark is useless against him! Thankfully he had a Weaksauce Weakness... or has he?
Took a Level in Kindness: The main characters, Frollo, Gaston, Panty and Stocking, are debatably nicer in the show then their canon counterparts are. This is especially the case for Gaston.
Villain Decay: Played for Laughs with Hitler, who goes from a formidable threat in Frollo Reads Mein Kampf to a Harmless Villain in his next major appearance. He's back on top of his game in Frollo Gets Flashed by a Gothic Lolita.