These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
Tear Jerker: There's a lot of them. Several of Yukiko's arguments with her father qualify. Especially A Reason You Suck Speech she gave out to him before saying, "I hate you." She even felt sadness when she remembered it.
Musaru's death - taking the bullet for his daughter
Several of the backstories are depressing.
Yukiko: She lost her dog and twin brother at a young age and her mother abandoned them. Actually, she was murdered as a message to Musaru not to defy the Shogun
Daichi/Kaori: Daichi was a loyal soldier to the Shogun until the Shogun decided he wanted Daichi's daughter, Kaori. When Daichi refused, the Shogun cut Kaori face - if he can't have her, no one will.
Michi: Lost her parents at an early age and was taken care of by an Uncle who stirred a rebellion and had to watch as her Uncle was forced to commit Seppuku.
Hana/Yoshi: Their father was an abusive alcoholic, the family being raised in constant fear - though Hana was spared direct assault. When their mother finally defied their father, he murdered her and tried to gouge Hana's eyes out before being slain by Yoshi. Since, the two siblings have been constantly impoverished and trying to find a way to survive
When Michi finally tries to rescue Aisha, she discovers that Aisha's been paralyzed from the neck down and kept alive by Guild machines. Quickly doubled when Aisha asks for a Mercy Kill
Love has very little success in the series. So far:
Yukiko and Hiro: Hiro is unable to leave his Lord's service while Yukiko wants nothing more than to kill the Shogun. Relationship ends when Hiro betrays Yukiko and Burru and Yukiko team up to tear Hiro's arm off and stab him. In Kinslayer, Hiro feels compelled to avenge his Lord on Yukiko but is quickly becomes clear that her betrayal towards him is his real motivation. Add in that Yukiko is carrying Hiro's children...
Yukiko and Kin: Kin loves Yukiko, Yukiko's feelings are mixed. They get together in Kinslayer but then Kin betrays the Kage
Yoshi and Jurou: Long-time couple until Yakuza torture Jorou to death