These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
Pretty much any character introduced for a one-off joke can be guaranteed to become a fan favourite, leading to many an Ascended Extra. Examples include Div, the Fruit Fucker 5000, Twisp and Catsby (also Springtime for Hitler) and the Broodax Imperiate.
SHUT YOUR PIE HOLE!
Tycho's niece, Anne, also known as Annarchy, won a poll by a landslide to determine which character to focus on as an arc during the authors' trip to Comic-Con 2011. Hence, Annservice.
Fan Dumb: The whole Sixth Slave controversy went on for MONTHS. Not helping this were groups like the "teamrape" trolls, who, among other things, sent pictures of mutilated and dead women to known rape victims in the fandom.
This strip, which is now accidentally brilliant because it makes perfect sense that anyone with knowledge of the future at the time it was published wouldn't remember that "Revolution" was the codename for the Wii rather than a huge historical event.
And this infamous strip, whose mockery of the bogus release date for Duke Nukem Forever was funny in 1999 when the game was a year and a half late, but only grew more so as the game grew two years, four years, eight years, nine years, only to loop back around to Hilarious in Hindsight when a demo of the game, now out of development hell, was released at PAX.
This strip became a lot funnier when a few years later Tycho actually did miss a portion of PAX due to the birth of his child.
Internet Backdraft: As widely respected as PA is, its subject matter choices and...let's say "irreverent" approach have produced a few.
The controversy over The Sixth Slave, which turned into a six-month internet war that resulted in boycotts of PAX and death threats from both ends.
A more insular version came back in 2006, when the guys mocked a webcomic documentary they found silly and pretentious, singling out Cat Garza—who had quite the fanbase. They came under a small amount of fire from said fanbase.
Thanks to Paul Christoforo's very public name and shame on the website, his face (taken from his Twitter account) quickly got associated with terrible customer service. His horrible emails - both in terms of content and grammar - supplied such memetic gems as 'Son Im 38 I wwebsite as on the internet when you were a sperm in your daddys balls and before it was the internet'.
In the more traditional sense of a meme, the word "bullshot".