These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
Pretty much any character introduced for a one-off joke can be guaranteed to become a fan favorite, leading to many an Ascended Extra. Examples include Div, the Fruit Fucker 5000, Twisp and Catsby (also Springtime for Hitler) and the Broodax Imperiate.
SHUT YOUR PIE HOLE!note Charlie's catchphrase
Tycho's niece, Anne, also known as Annarchy, won a poll by a landslide to determine which character to focus on as an arc during the authors' trip to Comic-Con 2011. Hence, Annservice.
Gabe shooting a death seeking Sonic in the head in "A Ring and a Prayer", in light of Sonic Boom: Rise of Lyric becoming an unprecedented critical and commercial disaster just days after the comic was posted.
This strip, which is now accidentally brilliant because it makes perfect sense that anyone with knowledge of the future at the time it was published wouldn't remember that "Revolution" was the codename for the Wii rather than a huge historical event.
And this infamous strip, whose mockery of the bogus release date for Duke Nukem Forever was funny in 1999 when the game was a year and a half late, but only grew more so as the game grew two years, four years, eight years, nine years, only to loop back around to Hilarious in Hindsight when a demo of the game, now out of development hell, was released at PAX.
This strip became a lot funnier when a few years later Tycho actually did miss a portion of PAX due to the birth of his child.
The 2014 Valentines' pins are heavy on the yay, what with the motif being Gabe and Tycho happily sitting together on a park(?) bench, the perspective making it seem as if their hands are touching, with a huge heart in the background.
Internet Backdraft: As widely respected as PA is, its subject matter choices and... let's say "irreverent" approach have produced a few.
The controversy over The Sixth Slave, which turned into a six-month internet war that resulted in boycotts of PAX and death threats from both ends.
A more insular version came back in 2006, when the guys mocked a webcomic documentary they found silly and pretentious, singling out Cat Garza—who had quite the fanbase. They came under a small amount of fire from said fanbase.
Thanks to Paul Christoforo's very public name and shame on the website, his face (taken from his Twitter account) quickly got associated with terrible customer service. His horrible emails - both in terms of content and grammar - supplied such memetic gems as 'Son Im 38 I wwebsite as on the internet when you were a sperm in your daddys balls and before it was the internet'.
In the more traditional sense of a meme, the word "bullshot".
The Bump, particularly the third and fourth panels.
They Changed It, Now It Sucks: In early 2003, the comic underwent a shift in art style to a somewhat sketchier look. This caused some initial backdraft, if mainly out of the suddenness of the change than anything else. These days, style changes to match the tone are considered the norm.
Viewer Videodisc Format Confusion: Div, the alcoholic DIVX player, is easily mistaken by newcomers who have not yet read the older strips (or have little to no knowledge on a DIVX player) for a DVD player.
On a similar note, the wombat symbol is thought to be a bear by people who haven't read the older strips either.