These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
Arc Fatigue: Until a while ago, most of the fans were complaining about Jo focusing too much on Dota 2 comics and rarely doing any other comics. With how long the Dota 2 comics have gone on, and Jo adding a comment pending attribute to his site, the rage has stopped and turned into silence.
Base Breaker: Some strips reference the My Little Pony: Friendship Is Magic series. Some of the base is "Alright! Welcome to the herd, Jo!" and the others are "Seriously? This is one of the last pony-free places on the Internet! Not you too!". And those are the milder comments. You can read the rest here.
Broken Base: There is a considerable rift between those who prefer Team Fortress 2 comics and those who prefer DOTA 2 comics which isn't helped by the fact that most of the gags for either will only make sense if you really know the games meaning a huge chunk of the base simply won't get the joke for either.
Growing the Beard: Originally started off completely textless in an attempt to be the antithesis of Ctrl+Alt+Del, and to the detriment of its humor. Because Tropes Are Not Bad, once the strip became a lot more lenient with allowing text (and as a result shifting from the emphasis on TF 2-tan Moe fanservice towards general gaming humor), it became a lot more consistently funny.
One of the rants Jo attaches to his strips had him trying to convince people that the Wii would be Nintendo's last home console. Barely a day later, many sources started claiming that Nintendo would be unveiling their new console at that E3 2011.
Cast your eyes on this comic, which was released about a day or two after the Uber Update. Now, have a look at one specific item that Valve added into Team Fortress 2 on the first of July, about a week after the Uber Update.
Memetic Mutation: Every comic will have at least one person put a "spy here!" photo note on some area of empty space, nearly every pointed finger will have a photo note of "POW! Haha!" and anything with a female will have a photo note that lays claim to the female (or just part of their body). Lines from the Meet the Team videos are also commonplace, and to a lesser extent, "Nerf Now, now with more trope.". Lampshaded by the author in this strip and this strip.
If any character's open hand is visible, somebody will probably leave a photo note saying "Slap my hand!" referencing the Spy asking for a high five.
What an Idiot: When Engie goes to star in her own game, she goes to Minette for advice. She does this on the grounds that Minette is still just an NPC, having not gotten enough fan votes to be added to Skullgirls as a playable character, and would be a good example of what not to do. While that might be a good idea, actually telling her that is not.