These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
Deadpool: I can take down any of those goody-two-shoes wimps!
Designer: The Hulk?
Deadpool: Okay, maybe not him, bu-
Designer: The Thing?
Deadpool: Sure! If he had a cold....
Deadpool: Look Poindexter...
Deadpool's simulation mission in the original has him claiming his origin is a mixture of Storm, the Hulk, and Thor's, and then further going on to infuriate Arcade by enjoying being in Murderworld. He even mentions in the main game that the only letdown of being there was that he did not get a T-shirt (while talking to Vision in the Sanctum Sanctorum).
Deadpool's unique conversations in MUA1 especially his talk with the Ancient One.
Game Breaker: One that was acknowledged and fixed: Deadpool's teleport in the first game. Essentially, it works by going for a spot a certain distance from Deadpool in the direction he's facing: if there's an area the player is allowed to go at that point, then Deadpool will instantly move to it. If not, he'll go as far as he can along that path. Elevation doesn't matter. Nothing in the way matters. Because of this, if used smartly enough this can be used to - in addition to getting places the player usually can't - skip whole areas of the level as the player is fully allowed to go through walls, Insurmountable Waist High Walls, voids and rivers, even burning wreckage as long as there is something to walk on on the other side. In the sequel, Deadpool's teleport was unsurprisingly changed so that it does not go through things the player is not allowed to go through, and the range is decreased to boot.
The sequel gives this status to Storm near the end, where the nanites allow enemies to share powers with each other. Their powers tend to be either telekinesis, fire or ice, the latter two being powers Storm is immune to, making a good lot of the challenge in the later missions including the final boss fight with Nanite-Fury.
Doctor Strange happens to have a spell that has a chance of... instantly killing any non-boss enemy in the game by turning them into a box. A box that can contain GOODIES.
Harsher in Hindsight: Deadpool's fight with the devs of the game has them threatening to make Deadpool DLC. This was soon before all of the DLC for the game would become impossible to unlock without console commands.
Iceman/Deadpool/Spider-Man/Human Torch: Why are [Iron Man and Captain America] so obsessed with each other, anyway? It's reaching "creepy ex" territory.
This, from 1:
Spider-Man: Hi, again. Shall we continue to discuss how wonderful Tony Stark is?
Narm: Penance in MUA 2. Also a fair chunk of Mephisto's Realm, with the use of "astral energy" instead of "soul" and Nightcrawler's Small Nein.
In the first Pro-Reg mission, as soon as you run into the White Star, one of them says in the whiniest voice imaginable: "It's a raid! Get to your positions!"
You know those big hulking... things you run into at the omega base? Seem kind of intimidating, don't they? Then you run into them again in Asgard, and they talk. And they sound exactly like you'd expect them to. Another enemy that suddenly talks in Asgard is the Clay Warrior. His "Halt! You Shall Not Pass!" might have been Bad Ass if you hadn't just spent a level one-shotting about two-hundred or so of his kind.
In the first game, one of Doctor Doom's victory phrases after defeating an enemy is "Finally! I have achieved my ultimate triumph!". When he says this after defeating Random Mook #284, it's either this or Mundane Made Awesome.
Nightmare Fuel - In the second game the Pro-Registration side is using mind control to control villains and force them to hunt heroes for them. During one of the missions you can find a audio clip of Reed Richards casually explaining the device to what's presumably a room full of young scientists, while the victims villains, who actually volunteered to join their side (albeit presumably for selfish reasons), fruitlessly scream in terror while they're forcibly injected with mind suppressing nanites and their minds are subjugated in the background. It's somewhat more terrifying than it sounds. Later, the playable villain characters talk hatefully about how it felt to lose their minds and individuality to the Fold and agree to join, no-strings attached, if only to make sure that that sort of thing can never happen to them again. It takes a lot to make Reed Richards the most terrifying character in a game that includes characters like Venom and the Green Goblin, who you instead feel sorry for, but there you go.
I dare you not to feel a little unpleasant hearing Venom's breathing slowing as he's sedated. And then again maybe 10 seconds later when an unidentified female villain is brought in to be injected, all the while screaming for the S.H.I.E.L.D. personnel: "No! Get away from me! No!"
Tier Induced Scrappy - Elektra and Blade in the first game, despite being featured on the box art. Not surprisingly, they are not playable at all in the sequel (save for Blade in the PS2, PSP, and Wii versions).
Unfortunate Implications: In the Wii version of the second game, you have to stop a non-super powered prison riot. All the prisoners you fight are black. Also, most of the infected Wakandan either act like savages panthers or use bows and axes. You would think that the country that's miles better than any other in technology would have the royal guards using high-tech guns (which you've probably seen in the comics).