These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
Nightmare Fuel: Several characters are gruesomely altered or killed outright. The giant room of vermin in Angkor Wat isn't very pleasant, either.
The sequence on the Incan Gold Ship was pretty unsettling too. When you first arrive on the ship, there's all of these people who think you're the king for some reason. The ship sets sail into the beautiful blue sea, and you eventually go take a nap. You have a strange dream involving your mother and the approaching comet, and when you wake up, everyone you had previously talked to is a decayed corpse. Then one of your childhood friends gets eaten by a sea monster.
The Jackal's death. He is set on fire by one of the Pyramid's sound-activated booby traps (which you triggered) and is slowly burned alive, crawling across the floor towards Kara until his body stops moving. And it's all completely onscreen.
Castoth, the first boss: The first major combat challenge you face, with multiple parts to worry about.
Jack and Silvana, the third boss: Married vampires, who have very good coordination and require you to pay close attention to their movements to avoid their attacks. And have a combination attack that is hard to dodge if you're not prepared for it at can knock of roughly 1/3 of your life in one shot.