These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
Ass Pull: Dr. Nefario and the Super-Serum Antidote
Award Snub: Despicable Me 2 dominated the 2014 Kids' Choice Awards winning "Favorite Voice from an Animated Movie" for Miranda Cosgrove and "Favorite Mobile App," but getting shut out of "Favorite Animated Movie" by Frozen. Yes, Frozen was a lot bigger than DM2 was, but still, it put a blemish on a despicable night of awards.
Ear Worm: "Another Irish Drinking Song" as sung by the minions.
Pharrell Williams' "Happy" which makes you sing and dance while walking on the streets.
Fan-Preferred Couple: Even though Antonio dumped Margo for another girl, leading to Margo's current "hatred" for boys, many fans have a different idea. Some fanfictions and music videos still pair up Antonio and Margo.
Hilarious in Hindsight: In the first film, Gru tries to keep his supervillain identity a secret by claiming he was a spy. Cut to this movie, and he is hired as a spy for the Anti-Villain league.
There's also the small gag when the girls try to set Gru up for online dating, and he claims he looks like a bit like Bruce Willis. What makes this scene hilarious is that Gru is voiced by Steve Carell, and both of the actors worked together on another movie in which Carell's character looked up to Willis' character.
Memetic Mutation: The minions' imitation of a fire engine's siren: "BEE-DO BEE-DO BEE-DO....."
The 24-hour music video of "Happy" spawned many covers from people around the world where they sing and dance along with the song. Its title is "Pharrell Williams – Happy – We Are From [name of the city]"
Nightmare Retardant: The Evil Minions. They look like deformed Build-A-Bears and sound like they're vomiting. Not exactly scary is it?
The Scrappy: Much like Gru, there's plenty of people who hate that Chicken.
Strangled by the Red String: Gru and Lucy don't show much attraction or chemistry for the first half of the film, and their feelings for each other seem to come out of nowhere for the sake of them being in love.
They Wasted a Perfectly Good Plot: Most of the villains from Despicable Me go unpunished - in fact, don't even appear. Mr. Perkins still presumably runs the Bank of Evil, Miss Hattie presumably still runs the orphanage and neither of them figure in the plot. The sequel instead focuses on a Love Interest for Gru.
It looks like Little Miss Badass Edith will finally get a moment in the spotlight to fight the Monster!Minion in her ninja costume... then Dr. Nefario shows up in the nick of time and injects it with anti-serum.
The Margo/Antonio subplot is immediately dropped after Gru goes Papa Wolf on Antonio. Antonio completely disappears from the plot after that.