These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
Crowning Moment of Heartwarming: When he's introduced, it's clear Eario desperately wants to be related to his hero, Mario, somehow. There's the obvious moment at the end of the Janitor of the Mushroom Kingdom arc when his son, Waluigi, invites him to his own family reunion, but there's also the fact that they are indeed related, though it takes some Fridge Brilliance to figure it out: according to Nintendo, Waluigi and Wario are brothers, and Mario and Wario are cousins, making Eario Mario's uncle. This can very quickly become a Tear Jerker if one realizes Eario will probably never know that, however.
"Funny Aneurysm" Moment: In the comics Matthew made to advertise Howard Phillip's Kickstarter project (accessible at the bottom of this page), one woman, angered that her kids are making her buy an expensiveNES, tells Howard "I hope you fail." Howard's Know-it-All project ended up failing to reach the necessary funding. 'Tis a shame, as it could have done wonders for the educational system.
Genius Bonus: The Turnabout Kirby arc becomes a bit more amusing if you know that John Kirby was the name of the lawyer who defended Nintendo from Universal's allegations that Donkey Kong infringed on their copyright.
Many parts of the Mushroom Kingdom Carol arc, particularly the eighth panel of this strip, become hilarious after the Mario cast's appearance in Fortune Street.
The joke in this comic is funny to begin with, but it becomes even more hilarious when you realize it's exactly what ended up happening.
This comic featured Kirby attempting Wii Fit. One of the next Super Smash Bros. characters turned out to be... a trainer from Wii Fit. And her intro trailer shows Kirby still having trouble with yoga poses.
Now even more hilarious taking in account that both of them are in the next game
This comic becomes even more hilarious when you realize that Matthew posted it hours before of the E3 trailer of the next Smash Bros, announcing the inclusion of the Animal Crossing Villager in the playable roster.
Either hilarious or tear-jerking depending on the reader, but this comic ends up being more true than expected after Mario fighting the villain solo in the first battle of Mario & Luigi: Dream Team turns out to be a dream of Luigi's.
"Hey look! I remembered to make Sonic's arms not-blue this time!" Sonics arms are now blue.
Rescued from the Scrappy Heap: Waluigi was originally created so that Wario could have a partner in Mario Tennis, and was disliked by critics and players alike. Years later, in comes this comic, turning Waluigi into a psychopathic Cloud Cuckoo Lander, and he quickly became one of the best characters in the strip.
And Eario, the janitor of the Mushroom Kingdom. He cleans and polishes (and refuels the bullet shooters) all for his idol Mario, and the day he got a coin from a block Mario hit was the happiest day of his life. Until he was reunited with his son(?), Waluigi.
Young Dedede in the Cocoon Academy Story Arc. First he gets into a food fight by accident, is sucked up by "Pinky," and gets detention for aforementioned food fight. When he gets home he has a terrible dinner (so says the mouseover text) and the next day he's sent to Headmaster Hand's office. And then later on, he thinks that he's lost "Pinky" as a result of fighting Dark Matter...
Luigi can't fantasizeHIMSELF being the hero. Also, Matthew saying he has low self confidence doesn't help...