"Funny Aneurysm" Moment: Also in "Bosko's Picture Show", there is a short scene of Adolf Hitler (in what is now considered the first time he's been caricatured in a Warner Bros. cartoon) chasing a caricature of Jimmy Durante with an axe. Although Durante was Catholic, not Jewish, it still stood that his most prominent (and thus, most comically exaggerated) feature was his large nose, which is associated with Jews.
Take That, Scrappy!: Bosko (and Buddy) shorts were run alongside other Looney Tunes shorts on Nickelodeon for a time. At some point they removed them and ADVERTISED IT, with an ad that went like: "See all your favorite characters! Bugs Bunny! Daffy Duck! Yosemite Sam! Porky Pig! ...and no Bosko! Sorry, Bosko!" This accompanied a short animation of Bosko in a closing Iris Out. No Bosko (or Buddy) cartoons were ever shown again.
Buddy's return in Animaniacs got him an entire episode that gave him this treatment.
Values Dissonance: The MGM shorts featuring him due to his redesign as a black human boy.
The early Looney Tunes entires gave him a stereotypical Southern drawl, but later shorts gave him a higher Mickey Mouse-esque voice.