These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
Ensemble Darkhorse: Some people consider Wacky Weasel to be this. There's a good amount of screenshots and fanart of him online despite being a one-shot villain.
TJ Finger also gets some of this for being a Badass.
In "The Day The Toon Stood Still," Toons are literally running out of time and fading away. Just before Lucky confronts the Big Bad, Bonkers tells him that if he doesn't hurry, all Toons everywhere will return to the drawing boards and eventually blank paper. Lucky is visibly shaken by this revelation and rushes into action. Moments later, when all hell breaks loose, you can see (for just a second or two) Lucky holding Bonkers and Fall-Apart Rabbit as they fearfully look on.
From "Basic Spraining," just two words: "Trust me."