Moral Event Horizon: It's established that whenever someone commits an unforgiveable crime, they are permanently marked with the Unversed eyes. Whenever a strong-hearted hero comes near them, they will get a glimpse of the eyes, and often a vision of the moment they crossed it, or anyone else they are allied with, as Aqua sees Courtney Gears torturing Clank, and Dr. Neo Cortex and N-Gin hatching a plan when she comes across the Tremaines.
Courtney Gears crosses this line when she attacks Blackwater City, which results in it's mayor's death. When Ratchet later confronts her, he sees the Unversed eyes while looking at her, confirming that she cannot be redeemed.
Lady Tremaine, meanwhile, crosses it with her daughters when they destroy Cinderella's dress after finding out that Ventus made it for her.
The Woobie: Oh, Nina. Sweet, little Nina. You're not even a villain, but your fath-er, uncle just had to pull you onto that hovercraft when Crash blew it up with a chicken.