Adam Dodd of Survival of the Fittest receives a horrified reaction from his friends after he shoots a comatose member of his group in the head. Needless to say, just about everybody there called him out on it.
During the final two, the other finalist Jack O'Connor brutally called him out on all of his murders throughout the game.
Dr. Horrible's Sing-Along Blog. The villain is the one to call the hero out on his idiocy. The hero had almost flattened the love interest by grandstanding.
After Agent Washington shoots Agent South in the Head he and the A.I. Delta are called out by Church to be "some cold motherfuckers". It takes half an episode for Church and Caboose to stop trying to calm him down with a very annoying "just calm down...we are all your friends...lower your weapon" way of speaking.
Oracle is a powerful telepath and one-time member of the Global Guardians superhero team. When Doctor Devastation hid a nerve gas bomb in a subway terminal in Washington, D.C., she used her telepathic powers to slowly turn his brain into cole slaw as she forcibly searched it for the bomb's location. While she saved the day, she was also expelled from the team for "gross violation of a suspect's rights".
As a post-script, as of the shut-down of the Global Guardians, Doctor Devastation was still a permanently hospitalized drooling vegetable.
The Salvation War takes time to note the problems the war against Heaven and Hell are going to cause. The first battlefield is now littered with tons unexploded shells, Earth's economy is a wreck, and people were shocked by the beating they gave to the demons.
Linkara's pet peeve is heroes who don't act like heroes. E.G., Frank Miller's All Star Batman and Robin, Neutro Number 1 and Superman at Earth's End.
Linkara finally gets this himself in Catwoman, Guardian of Gotham #2.
By seasons two and three of Marble Hornets, Jay is regular getting chewed out for his Genre Blindness, stalking, and focus on filming everything and putting it on YouTube instead of helping people.
In Strange Aeons, Arron gets chewed out by Nick for filming his most private moments and putting them on his channel.
Interlude 2 has Panacea calling out Glory Girl for using excessive force during her fights.
Chapter 18.3 has Clockblocker listing just how many bad things Skitter has done, that despite being claimed for the greater good, had farther reaching terrible consequences.
Chapter 20.5 has Taylor calling out Dragon and Defiant for breaking the unspoken superhero/supervillain rules by outing her secret identity in front of a cafeteria full of high school students — a maneuver which, incidentally, could have put a lot of civilians in danger. Her arguments are actually compelling enough that a third of the students join her and help her escape from the superheroes.
Speck 30.1 has Tattletale calling Taylor out on her inability to trust her friends and her tendency to approach negotiation by holding a figurative gun to people's heads.
In Double Rainboom, Twilight, and pretty much the audience's reaction to Rainbow Dash completely destroying Ponyville.
In the "Cannon Ball Run" episode of Achievement Hunter's Let's PlayGrand Theft Auto IV, Gavin had continuously messed up Team Lads' attempts to win in a round of "Cops n' Crooks", prompting Michael to ask what's going through his brain. When Gavin gives him the reasoning - that he was actively losing because they just kept on winning and thus he felt it wasn't fun - it pissed Michael off enough that he temporarily tossed him off of Team Lads.
In "Ryan's Heist" (Let's Play Grand Theft Auto V), Michael chews out Ryan for killing Geoff (though that was an accident) and trying to kill Ray (that one wasn't).
Santa: You been a naughty boy! You brought a plague of frogs! You best arrest yourself, you broke Your own law! Or was there something in Rule 6 I didn't understand? My list says "killed Egyptian dude, buried him in sand".
Moses: You ain't a saint, you a slaver like a Pharaoh in the snow. Stop with the unpaid labour, and let my little people go!
Jim: Let me mention, I'm impressed by all the vision that it took/For you to sign your name... Kermit the Frog: On all of Jack Kirby's comic books!
In their clash, Frederick Douglass spends near his entire rap calling Thomas Jefferson a Hypocrite for actively partaking in the African-American slave trade after fighting a war of liberation. Jefferson has no real comeback for it.
In "A New Planet & Antimatter!" by Matthew Santoro, Matthew dies and goes to heaven. St. Peter calls him out for being mean to his clone, Hugo.
Whateley Universe: Beltane makes a very harsh comment to Ayla that completely disregards everything he went through (the comment was 'Yes, yes, I'm sure it was all very traumatic, now keep up, we've got a schedule to meet!'). While she didn't know what Ayla had gone through, that's still a pretty brutal thing to say, especially coming from one TG to another. While Ayla didn't call her out on it at the time, he still gets annoyed thinking back on it, and wants to be a tour guide at least partially so he can do a better job in that respect than she did.
In the Marvel Funko Short "Spellbound", Iron Man is able to rescue Spider-Man from Loki's spell by taking away his staff. Spidey decides to get revenge with the staff by... making his act like a chicken. Spidey laughs at Loki, but Iron Man shakes his head disappointingly and asks for the staff back, making Spidey hang his head in shame.