Adam Dodd of Survival of the Fittest receives a horrified reaction from his friends after he shoots a comatose member of his group in the head. Needless to say, just about everybody there called him out on it.
Not to mention that during the final two, the other finalist Jack O'Connor brutally called him out on all of his murders throughout the game.
Doctor Horrible's Sing-Along Blog. The villain is the one to call the hero out on his idiocy. The hero had almost flattened the love interest by grandstanding.
Red vs. Blue: After Agent Washington shot Agent South in the Head he and the A.I. Delta are called out by Church to be "some cold motherfuckers". It takes half an episode to stop Church and Caboose trying to calm him down with very annoying "just calm down...we are all your friends...lower your weapon" way of speaking.
Oracle is a powerful telepath and one-time member of the Global Guardians superhero team. When Doctor Devastation hid a nerve gas bomb in a subway terminal in Washington, D.C., she used her telepathic powers to slowly turn his brain into cole slaw as she forcibly searched it for the bomb's location. While she saved the day, she was also expelled from the team for "gross violation of a suspect's rights".
As a post-script, as of the shut-down of the Global Guardians, Doctor Devastation was still a permanently hospitalized drooling vegetable.
There was this one time Osaka accidentally shot Irene in the foot (with a very powerful gun, to boot) and everyone called her out on it.
A comparatively minor example: In TV Tropes Will Ruin Your Life, Joy eventually calls Chris out for mocking Dash one time too many.
The Salvation War takes time to note the problems the war against Heaven and Hell are going to cause. The first battlefield is now littered with tons unexploded shells, Earth's economy is a wreck, and people were shocked by the beating they gave to the demons.
