"Yes, evil comes in many forms, whether it be a man-eating cow or Joseph Stalin, but you can't let the package hide the pudding! Evil is just plain bad! You don't cotton to it. You gotta smack it in the nose with the rolled-up newspaper of goodness! Bad dog! Bad dog!"
There's also Belgian superheroine Eclair, a one-shot character from "The Tick vs. Europe." She is more androgynous-looking than Jungle Janet, though (for instance, compare her to her more traditionally feminine sidekick Blitzen), but this is never commented on by anyone and Die Fledermaus even hits on her (to no avail).
Anal Probing: Subverted. When The Tick is abducted by aliens, he is enthusiastic about being probed to expand interstellar knowledge, but it turns out the aliens actually want him to help defeat their enemies. The thought of probing him never crossed their minds. When The Tick finally goads them into "probing" him they do so by tapping an electrical device against his elbow. He is sorely disappointed.
Animal-Themed Superbeing: The Tick... even if he doesn't actually suck blood. Also, Arthur is not a bunny, he's a moth (At least he could fly like one with his folding wings).
Not to mention Sewer Urchin, Die Fledermaus, The Ant, Man-Eating Cow, Bumbling Bee, Caped Cod, Portuguese Man-of-War, Cockroach, The Praying Mantis, Blowfish Avenger, Caped Chameleon, Captain Lemming, Fishboy, The Angry Red Herring, The Fin, Octo-Raymond, Shiela Eel, and more.
Anti-Villain: One episode had the mascot for an ice cream company, Uncle Creamy, mutated by its toxic product and literally turning into his costume. He's seen as the villain for most of the episode except by the Flying Squirrel, and eventually he reveals that he was attacking the company because he was trying to reveal that their ice cream is made from artificial sweetener and toxic waste.
Ax-Crazy: The Evil Midnight Bomber What Bombs at Midnight.
Badass Grandpa: The retired superheroes from "Grandpa Wore Tights," who despite their advanced age and failing powers, put up quite a fight against The Human Ton and Handy, and actually managed to defeat him.
This is averted in the Colombian Spanish dub, since they translated his name literally from German to Spanish and it's translated as La Rata Voladora (The Flying Rat).
Also, the Fortissimo Brothers from "The Tick vs. Europe" are the superstrong henchmen of a musical-themed villain. Fortissimo is a musical term meaning "very loud" in Italian, but it has a second meaning: "very strong".
Body Horror: "ARTHUR! My mustache is touching my brain!" Actually possible- a little bit of your brain actually does protrude into your sinuses (that's how you can smell). Also, The Tick's transformation in "Coach Fussel's Lament."
In the animated episode "Armless But Not Harmless," the Tick and Arthur are struck by a ray that causes their arms to fall off. The Tick takes his sudden debrachiation in stride. Arthur... not so much.
Tick:[clears throat] All right... uh... this is quite a pickle. But no reason to panic, Arthur.
Arthur: [long, anguished scream]
Tick: [spots a phone booth] Ah... come along, Arthur! We'll call for assistance!
Arthur: [screams again]
Not to mention the various, Dick Tracy esque villains, such as Zipperneck, who has a zipper on his neck that he can pull down to expose the inside of his throat to horrify his opponents, Chairface Chippendale, Bolthead and countless others. Also worthy of mention is The Deadly Bulb/Pig-Leg, who for some unknown reason has a live pig in place of his right leg. The pig is perfectly healthy and acts like a normal pig, but being attached to eachother leads to some strife.
Bunny-Ears Lawyer: Arthur is pretty competent in a variety of things, once you look past he's in a giant moth suit all the time.
Averted in Arthur's backstory in multiple adaptations: he attempted to wear his moth suit all the time at his accounting position, and this bothered the higher-ups enough that he was "encouraged" to take a leave of absence and get psychiatric help.
The Tick himself qualifies, as well. When compared to most of the other "heroes" in The City, at least The Tick has the drive and focus to get the job done, most of the citys other heroes, with the exception of American Maid and, when it comes to sewer-related crimes, Sewer Urchin, are either too weak, or too cowardly to be particularly effective.
Butt Monkey: It seems that the Moon itself is this in the animated series. It's one thing having Chairface Chippendale using a laser to partially carve his name on it but a Galactus expy took a bite of it. So everytime something bad happens to the moon it stays this way thoughout the entire series. Lampshaded in the series' guidebook when the Tick responds "Hasn't the Moon suffered enough already?"
Captain Ersatz: All over the place. The live action series had them for the major characters.
In Transformers Animated, of all places, The Tick gets one. Sentinel Prime is voiced by Townsend Coleman, resembles the Tick (he's large, blue and has a lantern jaw and antennae) and even speaks like the Tick at times. The biggest difference being that Sentinel is a Jerkass instead of a Cloudcuckoolander.
The City: Called, oddly enough, "The City." It's said to have been poorly Anglicized from the French "Les Citrons" (The Lemons). Apparently early French explorers found a large quantity of some kind of fruit in the area.
Clark Kenting: Clark Oppenheimer's glasses and The Tick's hypnotic tie.
Closer to Earth: American Maid, compared to Die Fliedermaus and most of the rest of the cast.
Clothes Make the Superman: The Carpeted Man. With his suit on, he possesses the awesome powers of static electricity and overheating. Without it... he is nothing.
Continuity Nod: Countless examples, in addition to the Deface of the Moon example below. In one case, Handy loses one of his eyes at the end of "The Tick vs. Arthur's Bank Account" and when Handy returns in "Grandpa Wore Tights," he's wearing an eyepatch.
Conservation of Ninjutsu: In the comics. The third issue is titled appropriately "Night of a Million Zillion Ninjas" and they're every bit as ineffective as you'd expect with such large numbers. Justified in part by the revelation that Ninja has become a cheap franchise.
Later the Tick successfully blows up the C, but is blown off the moon before he can complete his job, leaving a "HA" on the moon. Later that episode, a Captain Ersatz of Galactus takes a bite out of the moon, which also remains.
In the video game adaptation, the laser will manage to spell out "Chairface" on the moon if the Tick doesn't destroy it in time. If "Chairface" is spelled out, the time will be up and you will lose a life and start over where you left off when you fought the laser.
Dirty Coward: Die Fleidermaus. Despite being a superhero we almost never see him fight crime, and for one very good reason: as soon as he sees a weapon he runs and hides.
Disney Death: In "The Tick vs. Dinosaur Neil", Arthur and the army think that the Tick is Swallowed Whole by a mutated Dinosaur Neil, though the "gulp" Neil makes indicates that it is not the Tick that he has swallowed, but rather the special aspirin pill that our blue hero tries getting him to swallow while struggling inside Neil's mouth, until he manages to get the aspirin down Neil's throat successfully. It's just that the army doesn't see the action inside Neil's mouth as much as we, the viewers, do; and just when they think they lost the Tick and are about to shoot Neil, our hero manages to open the dinosaur's mouth and escape unharmed (though covered in saliva) in front of everyone.
Genius Ditz: The Tick may be very, very ditzy in just about everything, but he has shown a certain level of philosophical thought in some cases. Especially so in the second episode of the live-action series, where he learns that everyone (even potatoes) can die, and after thinking about it for about a minute, gives Arthur a motivating speech that actually makes sense.
Early-Bird Cameo: In one issue of the comic book, a mysterious female figure wearing a flying suit identical to Arthur's appears in a few panels. The comic ended before her story was revealed, but she later appeared in the animated series as Carmelita Vatos, daughter of the suit's inventor and (eventually) Arthur's love interest.
Arthur can be spotted flying around far in the distance before he was formally introduced in the comic.
Early-Installment Weirdness: Most fans know The City as a place overcrowded with crazy heroes and villains. In the original comic, the City is shown to be a very boring place for a crime fighter, while New York is crowded with heroes.
Evil Old Folks: The Terror, a crazy old has-been supervillain who used to be buddies with Stalin. Back in the day he used to be a credible threat; these days, not so much, but he still gives Arthur and The Tick a good go of it.
Evil Plan: Parodied in the pilot episode, when the heroes catch one of the Idea Men and demand he tells them his scheme:
Idea Man: Well, we thought we'd steal a lot of money, and then we'd be rich, and we wouldn't have to work anymore!
Eye Scream: One of the retired old heroes from "Grandpa Wore Tights", The Visual Eye, had the power to eject his eyeballs from their sockets with the phrase "Rocket From The Socket", and fly them around for scouting purposes. In his old age, he can no longer control them as well, and when he sends them out, they simply flop to the floor, where the Human Ton ends up slipping on them like a pair of marbles.
"Fantastic Voyage" Plot: Without as much shrinking, though, as the Tick needs to get into Dinosaur Neil's mouth and get him to swallow the aspirin in order to neutralize the dinosaur DNA in his body.
Feghoot: The episode "The Tick Loves Santa." Dear GOD, the episode "The Tick Loves Santa."
Felony Misdemeanor: The Dreaded Infinity Ball, the most powerful weapon of the Hey Empire. Lampshaded by The Tick: "We have things like those on earth. We knock them into little pockets with sticks."
In the comic, the Tick and Arthur are basically in a (nameless) team, where they have similar roles to the cartoon. They're joined by Rubberduck, the Caped Cod, and Bumbling Bee as, respectively, the Lancer, Big Guy, and Chick.
Flanderization: Just compare The Tick from his earliest appearances (among other things, he actively hopes to avoid Ho Yay) to some of his later adaptations.
God Guise: Of sorts, as other inmates of the Evanston Asylum that escaped when The Tick did start worshiping him, forming the Mystic Order of Arachnid Vigilance. Which then underwent Defictionalization as The Tick's fanclub.
Hand Puppet: Handy, the Human Ton's good pal. "Read a book!"
Heroic BSOD: In the comic story "The Night of a Million Zillion Ninjas," The Tick's ally Oedipus gets stabbed by two of the ninjas, and she ends up needing medical attention. The Tick completely drops his usual demeanor- he silently (and ruthlessly) defeats the two ninjas and forlornly carries her unconscious body, while whispering "This isn't supposed to happen." After handing Oedipus to paramedics, The Tick goes into a full-blown psychotic breakdown, complete with delusions of buildings questioning his worth, and he tries to deal with it by single-handedly destroying the theme park base that the ninjas built.
Large Ham: Pretty much anytime that Tick opens his mouth. The scary part? His villains are often even bigger hams than he is! Done, as just about everything else in the franchise, as an Affectionate Parody of super hero comics.)
Limited Wardrobe: Tick and Arthur, and most of the other superheroes for that matter, are NEVER seen without their costumes, to the point where they usually sleep in them. In Tick's case, its debatable if it comes off at all. Arthur was seen without the bottom half of his suit in "Little Wooden Boy", after Swiss mercenaries stole his pants.
Mad Bomber: The Evil Midnight Bomber What Bombs At Midnight (yes, that is his full title). "Yeah, baby!"
Mad Scientist: The comic and cartoon being what it is, it has these guys coming out of its ears, but the most notable ones are the evil Dr. Chrome Dome, Brainchild and the mostly good but somewhat quirky Professor Vatos, the creator of Arthur's flying suit, as well as Dr Mung Mung, a less villainous take on the Yellow Peril, and the trouser-obsessed Dr Strangepants.
Magic Pants: Subverted and lampshaded in "The Tick vs. Dinosaur Neil," where Dinosaur Neil's monstrous transformation renders him completely nude, causing a group of top scientists outside of The City to attempt to clothe him with the largest pair of pants.
Well, they would. They carefully studied the situation, and came to the conclusion that the fact that he was pantsless was Neil's beef.
Mood Whiplash: The comic buzzes along as a pretty silly send-up of superhero comics... up until one of The Tick's allies gets stabbed and is sent to the hospital. It's then that the reader is reminded that The Tick is a recent escapee from a mental hospital, and he proceeds to have the kind of breakdown (fortunately focused on the bad guys' lair) that only a mental patient with Super Strength could have- he spends at most a couple of hours to completely destroy an entire theme park, single-handedly. It's about a full issue before The Tick (character, not the comic) manages to stop being so serious.
Only Sane Man: American Maid. She's likely the closest thing to a competant hero here.
Arthur counts as well to an extent. Okay, the guy did want to become a hero on the first place, and was fired from his old deak job by showing up to the office in-costume, but next to the Tick, well...
Paper-Thin Disguise: Often used by Tick and Arthur, who will usually just wear obvious disguises over their costumes, and in one episode, Die Fledermaus "disguises" himself and the Sewer Urchin with the contents of a potting shed (in order to get into a villainous auction).
Die Fledermaus: Greetings. I am The Rake. And my companion here is Buckethead.
Sewer Urchin: Can't see! What's goin' on? Definitely got a bucket on my head. Yeah.
Pulling Themselves Together: The Living Doll is, as his catchphrase goes, "full of tinier men." Of course, that means that once the fighting's over he has to round himself up and make sure he's got all of them in the right order.
Spoof Aesop: "You know, though today was the worst day of my life, I learned many things. First, the world looks a lot different when you're six inches tall and covered with feathers. Second, two heads are definitely not better than one. And finally, you can lay eggs and still feel like a man."
Straw Nihilist: The Heys, an alien race that coincidentally happen to look exactly like Arthur, who have a species wide obsession with non-existence and the lack of purpose of life.
This Looks Like a Job for Aquaman: Sewer Urchin, normally somewhat useless and meek, becomes a Badass in the episode where they have to battle his sewer-based archnemesis. Here, he is largely unflappable with just the right tools for the job and a pretty cool base of operations.
Arthur:[sotto voce] Who knew Sewer Urchin of all people would be this cool?
Sewer Urchin:[revealing that he heard Arthur just fine] Down here I'm the apotheosis of cool.
Title Montage: With most of the clips coming from "The Tick vs. Chairface Chippendale", "The Tick vs. Dinosaur Neil", "The Tick vs. The Idea Men", and "The Tick vs. Mr. Mental".
We Want Our Jerk Back: With the villains bringing him back. "We've got to rescue the Blue Tick, who is also mad, but in a much more goal-oriented way."
What Kind of Lame Power Is xxx, Anyway? Many heroes and villains have weird/marginal abilities: The Indigestible Man, Pig Leg/The Deadly Bulb, Bi-Polar Bear, the Caped Chameleon, the Carpeted Man, Four-Legged Man, Baby Boomerangutan, loads more...
Tick: Oh, Arthur! A little spit never hurt anyone. (Beat) Except for Edible Lad. Poor guy.
Apparently there was also a "Tommy Gun" that did something similar.
"The Tick vs. Europe" was this mixed in with Visual Pun and Bilingual Bonus. The Belgian superhero-sidekick duo of Eclair and Blitzen both have names referring to lightning (éclair is French for "(flash of) lightning", blitzen is derived from the Germanic word blitz), with Eclair actually being a lightning user. On top of this, an éclair is a French pastry (which is the first thing Tick thinks when he hears her name). Naturally, Eclair and Arthur have to take down a villain known as The Breadmaster.