Autocannibalism: In one short, Itchy (disguised as a restaurant employee) serves Scratchy his own belly. The unknowing Scratchy's attempt at eating it only results in the bits of it he swallows popping back out of a hole cut in it.
Early Installment Weirdness: "Manhattan Madness", a cartoon created entirely by Chester J. Lampwick, only features Itchy and had him visiting Manhattan to attack people. "That Happy Cat", meanwhile, is a solo Scratchy effort by Roger Myers Sr. with no plot whatsoever. Then Myers stole Itchy and his formula and used them in "Steamboat Itchy", and the rest is history.
Krusty: Wow! They'll never let us show that again, not in a million years!
Also, diePod Slaylist, which is from season 20, but is not listed in the ultimate episode guide of the Simpsons seasons 1-20.
Moral Myopia: Itchy constantly abuses, torments, mutilates, and murders Scratchy and enjoys it all the while. But if someone else takes enjoyment out of watching Scratchy be in pain, he gets all up in arms.
The Movie: In-story, the show had a movie released in theaters and either had a sequel or rerelease at the start of The Simpsons Movie.
Mr. Alt Disney: Itchy and Scratchy's inventornote well, Scratchy's inventor at least, Roger Myers Sr, is a thinly disguised spoof of Walt Disney with his theme park and the urban legends surrounding his antisemitism and being cryogenically frozen after his death.
Re Tool: Into a Lighter and Softer show. It was changed back when Marge Simpson, who started the protests against the show's violence, had a wholly different opinion on Michelangelo's David (which was on a coast-to-coast tour of the United States at the time) from the rest of the protesters.
The first cartoon in 1928, "That Happy Cat" only showed Scratchy walking down the street and whistling. Unsurprisingly, it wasn't a success, and the series only caught on when Itchy and violence were added (read: stolen from Chester J. Lampwick).
Shoo Out The New Guy: A character named "Poochie", voiced by Homer, was introduced, gelled horribly with the show, and was dumped hastily out of the show.
Shoot The Television: The short "Little Barbershop of Horrors", "written" by Abraham Simpson, ends with Scratchy's head (now just a skull) going through a ceiling and into Elvis Presley's television set. Elvis promptly produces a revolver and shoots Scratchy's skull through the TV.
Elvis: Aww, this show ain't no good! *BANG!*
Shout Out: Many, to various classic cartoons. A notiable one is Itchy having the subtitle of The Lucky Mouse in his first, lost cartoon, referring of course to Oswald the Lucky Rabbit
Take That: In response to Marge Simpson's protests on the show, there was an episode featuring a squirrel with Marge's blueBeehive Hairdo trying to break off Itchy and Scratchy's fighting... only to get killed by them.
Roger Myers Sr is a very unflattering portrayal of Walt Disney. The backstory of the I&S studio is a mishmash of conspiracy theories about the Disney Company.
Team Rocket Wins: The 'lost' episode where Scrachy finally gets revenge on Itchy by running him down with a combine harvester.
A storyboarded episode had Itchy steal Scratchy's pie, so Scratchy threw him in a vat of acid. Unfortunately, this was during a massive censorship push, so if they went with Marge's suggestion, Itchy would have shared the pie. Which he stole.
"Burning Down The Mouse" is apparently the only aired episode where Scratchy wins, by blowing Itchy up with a large arsenal of explosives. Unfortunately, the viewers never get to see the end, because one of Homer's college roommates who are crashing at his house unplugs the TV and doesn't plug it back in until it's over, where the TV just turns back on in time to see a huge mushroom cloud and "The End" superimposed over the shot (followed by Krusty declaring that "They'll never let us air that again! Not in a million years!").
Vitriolic Best Buds: Scratchy continues to hang around with Itchy, regardless of the constant attacks.
Wartime Cartoon: In one episode, we see a clip of an untitled WWII-era short, where a very Tom And Jerry-esque Itchy and Scratchy team up to torment and kill Hitler. Immediately afterwards, Itchy turns on Scratchy and cuts his head off, joined by FDR to kick the bodies.
Roger Myers, Sr.'s other wartime contribution, "Nazi Supermen are Our Superiors", was not as well received.