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In the webcomic , mad scientist Dr. Lupin "Wolf" Madblood and his 15,000 robot duplicates spend a Narbonic week of strips singing the "Madblood Battle Anthem".
Richard from has a Looking for Group very... touching song from the upcoming animated movie.
In an in-comic example, Jason of asks Davan to play Judas Iscariot in his deliberately provocative play, Something Positive Nailed! Davan's big musical number is called "You're The Son Of God, But I'm A Son Of A Bitch".
The main villain of 's Homestuck Show Within a Show Squiddles!, Skipper Plumbthroat, gets Catchyegrabber and Plumbthroat Gives Chase in the Squiddles! album.
Although mostly lyric-less, Homestuck's main villain proper, Jack Noir, has the very catchy and uptempo theme song - Black, which starts off slow and simple, then builds into a grand production.
There's also a whole album of Villain Songs in the form of The Felt, for the resident time-travelling Gang of Hats. Special mention must go to the baroque, atmospheric Scratch (for Doc Scratch) and the tense, slow-burning majesty of English (for Lord English), which later appeared in the comic itself as part of The Reveal.
The entire Midnight Crew also has a cover of I'm a Member of the Midnight Crew by Eddie Morton as their theme, a sample of which was used in the afformentioned song Black.
Meenah and her counterpart The Condesce get a non-vocal one called "Hate You". While there are no lyrics, it has a nice beat and even ends with the two of them laughing evilly!
has Brawl in the Family "A Simple Request", sung by Ganondorf about what he wants for Christmas: the Triforce.
There's also "The Minion Song", sung by Bowser Koopa, lamenting the many deaths of various videogame common enemies and chastising the heroes for their slaughtering them wholesale.
The Good Witch has "Things Will be Different." This is a rather unusual Villain Song in that it is both very upbeat and sung by the comic's protagonist. (Justified since she's a Villain Protagonist.) The song focuses on Angel's happiness that she is free from her previous life as a transsexual male who was ostracized by everyone in the town, and how great life will be now that she has infinite power. Which is to say, great for her, as she is also ruining the lives of everyone else. Throughout the song, she carelessly transforms innocent bystanders into random and bizarre things, and hypnotizes the others into singing with her. Ultimately, it foreshadows the selfish and arrogant bitch she'll eventually become.
Evil Plan The Webcomic has a theme for Mad Scientist Kinesis, by Andrew of Songs To Wearpants To.
Fraud from A Loonatic's Tale gets one in the chapter "Fraud's Day Out," set to the tune of Voltaire's Villain Song Brains!