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Keiji Tanaka of did this with "Like (something) and stuff". A Survival of the Fittest lot. As in 'At least once in almost every sentence,' a lot.
In , Zaboo often ends his sentences by taking one of the words from the sentence he just spoke and tagging on 'd. " The Guild I know every floor plan of every place where you've ever lived... floor-plan'd."
The Kobolds of often say "kobold" when they're talking. Or for (seemingly) no reason. All a person has to do to speak the "language" of the Kobolds is to say nothing but "kobold".
Unforgotten Realms Slowbeef during the second Kaizo Mario World Retsupurae and the 1st I Wanna Be The Guy Retsupurae. Here are the tics:
In , the ridiculously stereotypical American Bandit Keith from Yu-Gi-Oh! The Abridged Series adds "In America!" to the end of most of his sentences. Parodied in the episode where he duels Joey; as Joey plays Copycat, he declares, "This lets me copy your Yu-Gi-Oh! Catch Phrase... in America!"
It was also parodied in Keith's second appearance, where during a duel between Joey and Zombie Boy Keith gloats how Joey will be defeated only for the latter to reply "Let me guess...'in America', right?" Keith defensively responds " I wasn't going to say that! ...(inamerica)."
Also, Kemo (Pegasus' henchman, known as the "Nameless Henchman" in the series) constantly begins his sentences with "Attention, Duelists!" and narrates his actions and moods in the third person - but replacing references to himself with his (spectacularly pointy) hair. For instance, "My hair is inviting you to enter the castle!" or "Attention, duelists! My hair is assaulting you!" At one point, Keith and Kemo run into each other...
Same with Mai Valentine, who refers to herself as "My breasts".
Tristan does the same thing with "My voice", though not nearly as often.
Joey very often goes "NYEH!" for no reason, as a sort of sound for all occasions. He himself has no idea he's doing it, and seems not to hear it at all, wondering what Bakura means when he references the Nyeh-sound.
Taken to extremes in one episode where, after being called out on his nyeh-ing, he then proceeds to sing the entire Abridged theme song in nyehs, ending with "Still not hearing it."
Agamemnon Tiberius Vacuum has a weird verbal tic in which his speech is sometimes slowed, sometimes sped up, sometimes even reversed.
Chuggaaconroy says "Whatever" in his Let's Plays a lot, often without him noticing. He tries to cut back on it, but hasn't succeeded.
Mitchell, in the MSF High Forums and , has an interesting one, in AIM chat and IRC. He tends to surround 'action' posts with '8action8'. This is due to a incorrectly working shift key, which doesn't register his attempt to post '*action*'.
MSF High IRC The Entity from Atop the Fourth Wall has a tendency to stretch out certain syllables. "Welcome to my Glory, hyuuuumaaaan...."
90's Kid also tends to start his sentences with some variation of the word "Dude", while Doctor Linksano has recently begun to trill his R's.
Oh man, look at that Raocow and his verbal tics. His tics are such a thing. That's pretty great. Hey there, guy.
Chester A Bum of Bum Reviews has a tendancy to insert 'ma' into the middle of words (e.g. "computer-ma-ter" and Nostalgia-ma Critic).
The Irate Gamer pretty much says "pretty much" and "Alright!" pretty much all the time. Alright!
Pretty much any tutorial video is going to include copious amounts of "Like so" whenever they finish doing what they just said they were going to do.
Minecraft In , Pinkie Pie uses "bro" as (1) her default form of addressing Ultra Fast Pony everyone, and (2) an all-purpose interjection.
In , Pinkie has "Yes" and Scootaloo has "Hoo!".
My Little Pony: The Mentally Advanced Series Let's Player Clement often says "But I digress" in his Let's Plays. Especially seen in his Sonic LPs.
Vaguely Recalling JoJo Empress says Chumimin at the end of her sentences, which is the same Kiai used by Tusk ACT 4 in Steel Ball Run. When Joseph beats her, he calls her a Chumimin.
note Death 13 uses "Rally Ho" in its sentences a lot.