Hey, It's That Guy!: There's Gene Autry himself, and Pat Buttram, who later did character voices in four Disney animated features, as well as the voice of Cactus Jake, but is probably best known as Mr. Haney. Of course, there's also The Skipper as the bad guy in a few episodes as well as Gene Autry's replacement sidekick, Tiny, in two episodes.
Lee Van Cleef guest starred in two episodes, both times as a henchman to the Big Bad of those episodes.
Denver Pyle, who played either a law-abiding sheriff or a conniving villain, later became known for his roles as Uncle Jesse and Uncle Bene.