Ascended Fanon: One of the biggest cases ever. Coulson's death being undone in numerous fanfics and such spawned this entire TV show.
Creator Couple: Jed Whedon (the younger brother of Joss Whedon) and his wife Maurissa Tancharoen are the showrunners of the series. note Joss himself has a significantly smaller role than most other Mutant Enemy projects due to being busy with The Avengers: Age of Ultron.
Jossed: When fans saw that the pilot involved an African American man with superpowers, many assumed that it was Luke Cage. Whedon, however, assured that it wasn't him. For good reason, because he's actually the Villain of the Week and later, Deathlock.
Name's the Same: "The Rising Tide", the hacktivist group Skye is part of, is also the name of a Real Life climate change activist group. The real life organisation Rising Tide is not pleased with the series using the name, since fans have confused them for some super hacker group (when in reality they're just a normal protest group).
"Melinda May" is also a song by Stephen Foster published in 1851.
Production Posse: The pilot episode has appearances by J. August Richards and Ron Glass, who played Gunn and Shepherd Book in Whedon's previous series. (See Hey, It's That Guy! above.)