This is based on opinion. Please don't list it on a work's trope example list.
Tear Jerker / You Can Play This!
Iron Liz once showed up on JDub's livestream to ask for personal and professional advice. She began talking about her depression and panic attacks, and confessed that she tried to kill herself on her eighteenth birthday. Justin then calmly (and almost casually) admitted that he'd tried it, too.
The discussion also prompted a number of people in the chat to come forward with stories of their own struggles with depression and/or suicide attempts. It's a lot more common than you'd think.
Becomes Harsher in Hindsight thanks to his death. Especially when Justin talks about how easy suicide is in the heat of the moment. And then states, "Wellbutrin saved my life." Ouch.
Joe's is especially difficult to watch. It's unspeakably jarring to see a normally energetic, happy-go-lucky guy red-eyed and inconsolable.
Spoony had a nice quiet moment at the end of his Lethal Games review; he produces a Wario hat, places it on the back of his chair, and silently departs, leaving the camera to linger on it. The hat is visible on the shelf behind him in subsequent reviews.