How does the series start? a symbolic shot of Evangeline walking through her life, eventually leaving everyone from the previous series behind, even Chachazero.
It's also a bit of a Tear Jerker on a meta level, due to the implication that most of the Negima cast is dead. If you were fan of the previous series, it's a punch in the gut to realize that a lot of the characters you grew so attached to aren't around anymore.
It also gets even worse when one remembers that Negima also shares a continuity with Love Hina and A.I. Love You, and that the characters from those manga are also dead and long gone. Essentially you come to realize that, pretty much everyone you've come to know and love are dead, and that the only thing left here are their memories.
Touta Konoe is later seen praying at the graves of his parents. Their gravestones are positioned on either side of Touta's grandfather's grave, Negi Springfield.
How does the reunion between Fate and Eva goes ? Not good, they start fighting seriously like in early Negima. It seems Fate bears great hatred towards Eva now.
Sayuka saying goodbye to Santa. Despite all the problems she caused, it's clear that she did everything because she thought it would make him happy.