This is based on opinion. Please don't list it on a work's trope example list.
Tear Jerker / Total Drama Letterz
When Quana is eliminated, Spider tries to eliminate himself to save his girlfriend. It doesn't work, and Quana is sent off, with Spider sadly watching her until the boat disappears into the horizion...
In the next episode, Barney breaks his leg during the Donkey Kong challenge...which leads to him being eliminated from the competition. Before he is eliminated, he and Lavender say their farewells.
Vinnie sacrificing himself so that Uzuri can stay in the competition.
When Nakia verbally attacked VayVay of her bisexuality, it hurt her so much, she broke down in tears.
Jimmy getting himself eliminated because he felt like it was time for him to go.