Scruffy looked so sad when Harold accidentally squashed him.
You gotta feel bad for Duncan, too. He really loved that spider.
Tyler's fear of chickens was Played for Laughs, but the poor guy was CRYING on his way to the Boat of Losers. Made even worse in by how his teammates make fun of him, Chris enforces his fear by filling the boat with chickens, and Lindsay started forgetting his name! That must REALLY screw up a guy, psychologically.
Alejandro's final fate in World Tour.
The ENTIRE World Tour finale can be considered a tearjerker. Heather spent three seasons being the bad girl nobody likes, having to go to great lengths the entire third season just to get some RESPECT. And finally, after all this time, not only does Cody say she's the good guy in the finale, she defeats her nemesis Alejandro and wins the game, with people CHEERING FOR HER and wanting her to win for once! So after gaining lots of Character Development the entire season and becoming a Defrosting Ice Queen, the writers show how little they care about Heather fans, and possibly the show itself, by making her lose the money anyway. She already chose money over love and now she has NOTHING. And in the version where Alejandro wins, Heather loses by making a very simple mistake (throwing Al's doll in the volcano). The heartbreak in Heather's face when she realizes that she made it this far and beat Alejandro just to lose to him via technicality is absolutely heart wrenching. All of this culminates in a massively depressing GainaxEnding once the volcano erupts. And, just in case the writers didn't make their point, the last shot of the season shows Heather about to get smashed by a smoldering boulder This was such a Downer Ending it's hard to watch. Chris even jokes about everyone dying due to the volcano explosion, prompting a search for new contestants in season four.
Mike's farewell to Zoey also counts, especially when he gave her a gold medal.
Everyone knew Scott eventually had to get his comeuppence in TDRI. But did he have to lose most of his body along with the ability to move or speak? Especially cruel in that after explaining what happened to Scott, Chris exclaims "But who cares? It's Scott!" while everybody else LAUGHS. Scott sheds a Single Tear, because that's all he can do.
He gets better in Total Drama Island All Stars, back to full health.
However, he's absolutely terrified of sharks now, to the point that he mentally blanks out when Chris forces him to fight Fang. Not only is it hard to watch, but it's also Played for Laughs...
For the first time in his life Mike is alone. His personalities are missing, he has no idea what's happening to his mind and it's terrifying him.
In 'Food Fright', Gwen is crying over the fact she can't make up with Courtney, admitting this is the only reason she returned to a show where she gets publicly and internationally humiliated plus risking life and limb more than once.
Not to mention after all she went through to get back in Courtney's good graces, the A-Type reveals in the confessional in "Sundae, Muddy Sundae" that she doesn't plan on taking Gwen to the finale liked they promised one another. When Mal reveals Courtney's diagram and subsequent plot, Gwen's not only rightfully ticked off, but saddened that her efforts were ultimately fruitless.
Courtney's guilt and apologies to Gwen after the above.
Zoey losing her faith in Mike after Duncan points out Mal could be his real personality and Mike is just an alternate.
Then there's the fact that she regains her trust in the next episode after he saves her from piranhas. That sounds great right? Not exactly. It was Mal that saved her and not Mike. To make things worse Mal only saved her to avoid suspicion. Zoey may be in really big trouble and if Mal tries something she won't even see it coming.
Manitoba Smith's fate? He needs to take Mike's dreams (here represented as clouds) and burns them away. Harsh.
Even worse, he had no problem with doing it even as Mike was watching he burned them as if he was getting the newspaper
Zoey's sadness throughout the episode, thinking that Mal had replaced Mike forever (luckily Mike returns!).
Zoey saying farewell to Mike while burying the necklace he gave her last season
Mike losing all his alternate personalities to save Zoey from Mal. At least they all willing did so after Mike initially refused to do so.
Even though he was a huge jerk seeing Mal fade away and merge with Mike while yelling to Mike about how it was his time after seeing Svetlana,Chester,Manitoba and Vito do the same thing was somewhat sad.
The fact that the eliminated All-Stars contestants, save for Gwen, Cameron, Alejandro, and Heather have seemingly floated away to oblivion in the balloons. Many fans only watched the finale in hopes of seeing their favorite eliminated competitors again, so it was quite a cruel slap to the face.