This is based on opinion. Please don't list it on a work's trope example list.
Tear Jerker / The Fugitive
Kimble's thoroughly haunting dream about his late wife, which starts off all dreamy and romantic, then abruptly segues into his desperate, futile attempts to revive her, then holding her in his arms as she died.
Helen Kimble's 911 call—her voice weak and shaky, her pleas for her husband to help her. A literal example of this trope, as Kimble is seen crying as it's played in court—where it's being used as evidence against him.
Helen's death in general. Richard just came home from a charity party, there were rose petals all over the stairs leading up to their room so it was implied that his wife was waiting for him to come home so they could have sex. Only for him to find her dead and Sykes still in the house.
Kimble breaking down during the interrogation as the full impact of what's happened (his wife has been murdered) and what's going to happen (he's being blamed for it) starts to hit him. The cops mocking, taunting tones really don't help.
Kimble pleading to Gerard, "I DIDN'T KILL MY WIFE!". The man is just desperate for somebody, anybody to believe him, and Gerard responds with his infamous "I don't care!" (makes the Meaningful Echo at the film's end that much more). For a split second, it looks as though Kimble is seriously contemplating killing Gerard, possible figuring he has nothing to live for anymore and that he might as well be executed for something.
Even with Richard's innocence proven and the guilty men caught, at the end of the day, his wife Helen is still dead, and nobody is going to be able to change that for Richard.