- Darwin in "The Third", realizing he's almost lost his best friend by being too focused on Tobias.
- Rocky in "The Pressure":
Gumball: You've had girlfriends before, right?
Rocky: Oh yeah, thousands of 'em.
Gumball: Well how do you get rid of them?
Rocky: Well usually I just close my eyes, and open my heart! And when I open my eyes again, they're gone...
- Gumball and Darwin's song in "The Gi" is surprisingly heartwrenching.
- Gumball starts crying near the end of the episode, realizing that everyone has been making fun of him the whole time.
- In "The Kiss":
Gumball: THERE IS NO HAPPY PLACE!
- "The Robot" has Gumball trying to sleep in his backyard and crying after Bobert, who assumes his identity, has won over his family.
- Rocky talking to Miss Simian in "The Ape":
Rocky: Seriously though, no one likes you Miss Simian, not even me! And I like everybody!
- "The Meddler":
Penny: Well... *sniff* T-thanks for being honest with me....
- When Gumball runs to his room after being ignored by his parents.
- Penny's reaction to Gumball denying that he likes her.
- "The Helmet" has a Disney Death of Gumball when he attempted to get his helmet that was about to fall into the "dumpster's crusher". It made Anais scream at the top of her lungs.
- Similarly, though the circumstances were funny, Gumball's reaction to Darwin's Disney Death in "The Phone" is fairly sad - he drops to his knees and starts sobbing.
- The fight scene from "The Words" where Gumball hurts the feelings of Darwin in one of the saddest moments in the show.
Gumball: You are not my brother! You are just a pet, who grew legs!
- The two make up but it's still extremely saddening to see. It reduces the two to tears and it's sad to see two friends who stick by each other through so much cry together.
- Gumball thinking he murdered Idaho in "The Bumpkin" and crying over it once Idaho's family buries him.
- Richard's song in "The Hero".
Gumball: He's gone... and I was laughing at him. Why didn't I tell him in spite of everything I was proud, Darwin. I was proud of him! If he were still here I would tell him, if he were still here-*Gumball notices Richard is right behind him* oh, you're here.
- When Gumball thinks Richard has fallen to his death.
- In "The Pony", Anais wants to spend time watching a movie with her brothers, but a series of events continually delays Gumball and Darwin from returning home from the DVD store. Anais is at first unamused, no doubt thinking that they've messed up somehow, as per usual, but she eventually begins to consider that they just genuinely don't want to hang out with her and it drives her to tears. She clearly loves her brothers, so seeing her crying because she thinks they don't care about her is heartbreaking.
- "The Voice" sheds some light on William. Poor guy, all he wanted to do was hang out with Gumball and Darwin.
- Near the ending of "The Kids", where Gumball and Darwin sing, opera style, about how they are growing up. They get better in the end... though they're not sure if that's better or worse.
Maybe it's for the betterNo one wants to stay a child foreverRemember the wonderful times we've hadThose happy thoughts now make me sadThey say it's a fadIt's just a new start,So why do I have this pain in my heart?Our childhood memories are getting hazy...
- Darwin trying to exile himself in "The Allergy".
- Penny freaking out and running away in "The Shell", despite Gumball trying to tell her that he likes her true form. Her dad's reaction doesn't help much as he didn't even bother to tone down his comments to the point of calling her own daughter a monster despite Gumball's best effort to calm Penny down.
Penny: Gumball! Please tell my father that the monster is going to live in the forest, with all the other beasts! (Flies away)
- Penny's reaction to her father also did not help make this scene better.
- Gumball's risking himself to protect Penny from harm's way even if it means having to break her heart by telling her she's nothing to him... just to prevent her from being hit by a truck by getting her small enough for them to duck under it. Seeing Penny's small form in Gumball's paws after being called nothing to him is saddening to see, even more so that Gumball only did it just to protect her as you can see from his injuries. She eventually knew but that doesn't make it less saddening.
- In "The Man", Richard reveals about his father that the last time he saw him was that he went out for milk 42 years ago. Richard thought he would come back eventually and even all these years, but even his family and kids knows that's not the case. In the end, he finally knows the truth and he let his tears out while Jojo comforted him. He actually grew up without a father which explains why Granny Jojo was so overprotective of him, as seen in "The Authority."
- "The Butterfly": The Alligator Woman telling Rocky that she's been saving herself for marriage for so long that she became depressed, couldn't find a job, had to live in a van under the freeway with her cats, and ate noting but squirrels and rust.
- "The Friend": The shark-headed monster was left alone to wander the streets in search of friends after his creator died (or, in his words, "didn't wake up") and Gumball, Darwin, and Anais trying to protect him from the police, only for the shark-headed monster to kill himself with cola and mints (Fortunately, he gets better, but still has to leave town because of what happened).
- The revelation that Anais has no friends and she threw a birthday party just so someone — anyone — could come.