This is based on opinion. Please don't list it on a work's trope example list.
Tear Jerker / Splatterhouse
In the end of the fifth stage of the original Splatterhouse from 1988, Rick's beloved Jennifer (roughly) uttered the words "Help, my darling.", and then mutates into a hideous clawed beast. It cackles out an insidious laughter before and while fighting Rick. During the fight, Jennifer reverts to her normal self and begs Rick to save her before the monster takes over again. After the battle, Jennifer tragically dies in Rick's arms.
All three of the bad endings of Splatterhouse 3. While Rick saves the world from the chaos the Terror Mask intended to unleash, he's still devastated by his failure to save his wife and/or son, and the Terror Mask twists the knife by reminding Rick that it will return as long as he is suffering.
Failing to save Jennifer in time while succeeding in saving David ends with Rick a single father having to explain to his son that his mother is dead.