Please don't list this on a work's page as a trope. Examples can go on the work's YMMV tab.
Tear Jerker: Sayonara Zetsubou Sensei
Even the normally over-the-top ridiculous Sayonara, Zetsubou-Sensei has one of these in Episode 11 of the second TV series, when the girls sadly explain what has driven them to murderous insanity. "But... he never chose any of us".
The ending of the eleventh episode of Sayonara, Zetsubou-Sensei is bad enough, as it moves completely away from the usual wacky and over-the-top tone. That's his entire class waiting for him outside the operating room. And if that isn't tearjerker-y enough for you, here's this AMV/parody ending.
