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Tear Jerker: Sakura Taisen
Sakura Taisen. Iris's backstory and her first ever birthday party in charge of the Hanagumi. Full stop, go for your Kleenex.
All of each Token Mini Moes's backstories are qualified for this. Aside from Iris, we have:
Coquelicot: An orphan who work at a circus performer.
Rikaretta/Rosita: Lost her father at young age and work as a bounty hunter since. Heck, she even live in an empty warehouse.
The final battle's first part in V. Nobunaga takes away Shinjiro's partners, one by one, ending with his love..."so long," indeed.
The ending to 4. It feels like a a goodbye to the entirety of 1-3. Yoneda's monologue doesn't help, especially when he sees his fallen comrades Yamazaki, Ayame, and Kazuma smiling at him. That part really got to this troper, especially after having just played 1 to 3.