- In the opening scene, Obi-Wan loses R4-P17, his Robot Buddy throughout Attack of the Clones. While it's small potatoes compared to what he goes through in the rest of the film, it's an easily-missed omen.
- When Anakin's stuck trying to decide whether he should pursue Mace Windu and Palpatine, he stares out the window of the Jedi High Council's meeting room, and so is Padmé from her residence, both thinking of each other. They don't speak, but their despair—especially Anakin's—is palpable, and Anakin finally sheds a Single Tear, knowing he's about to make a decision that will change the galaxy's fate, all out of love for his wife, before leaving.
- At the spaceport before Obi-Wan leaves to pursue Grievous, Anakin admits he hasn't appreciated Obi-Wan's training and is dearly forgiven for it. The next time they meet, they're fighting each other in the galaxy's own living hell.
- Throughout the film, Anakin goes to so many lengths to remain true to the Jedi—when he talks to Yoda about his visions of Padmé's death, Yoda just tells him to let go of what he fears to lose, which he can't because Palpatine keeps dangling the prospect of saving her in front of him.
- Order 66. After all the Jedi's struggles together with their troops, they can see the Clone Wars' end in sight... until their soldiers, brothers-in-arms, and friends all have their blasters pointed at them. Everyone they've ever known from childhood is being shot down as enemies of both droids and clones. The Chosen One is now murdering children, and the Sith lord they've been looking for all this time is the Supreme Chancellor. In mere seconds, the Jedi went from war heroes to hated villains.
- When Ki-Adi-Mundi is about to get shot, he has just enough time to sink in how alone he is on that battlefield, betrayed by his own troops.
- When the music reaches its peak, Yoda drops his walking stick in pain. He's just felt the deaths of every single one of his Jedi Knights through the Force.
- Even with the inhibitor chips, which are a story all their own, some clones resisted and gave their lives to prevent more deaths but died in the process, and others were horribly traumatized.
- One Padawan kid (played by George Lucas's son Jett) tries to escape during the commotion and fights off the clone troopers pursuing him, but gets shot anyway. Bail Organa witnesses the whole scene, and is helpless to prevent it.
- "Master Skywalker, there are too many of them. What are we going to do?" This is the first time someone wholeheartedly addressed Anakin as "Master". The look on his face sells it; he doesn't want to do it but feels he has no other choice.
- Padmé weeps in her apartment, seeing the Jedi Temple on fire, and she realizes that everything she worked for in her life—her marriage, democracy, the Republic, her people, the Jedi and her children—is literally going up in flames.
- Yoda tries to stop Obi-Wan from watching the Jedi Temple's security feed, to protect him from the knowledge that Anakin killed the Jedi there—then they watch it, and Obi-Wan is horrified.
Obi-Wan: Send me to kill the Emperor. I will not kill Anakin.Yoda: Twisted by the dark side, young Skywalker has become. The boy you trained, gone he is—consumed by Darth Vader.
- When Obi-Wan meets Padmé there the following day, at first she can't believe that Anakin could've done what he did while Obi-Wan is still grief-stricken and trying to cope with the burden of what he has to do. The moment he realizes the secret Anakin's been keeping for so long really shows how much he's wrecked the lives of those around him.
"Anakin's the father, isn't he? ...I'm so sorry."
- The very next scene shows Anakin, after his relentless murder of the Separatist Council, weeping in utter and complete self-loathing. He knows what he's become, but has no hope for redemption.
- When Padmé meets with Anakin, to try and talk sense back into him, she ends up Force-choked by the only person she loved more than anyone else in the galaxy, because he wouldn't listen to her pleas.
- Every time Darth Vader Force-chokes someone, he's reliving one of the worst, vilest moments of his life.
- After his brutal duel with Anakin on Mustafar, Obi-Wan throws a Hail Mary in a last-ditch attempt to make him see the error of his ways. It fails.
Obi-Wan: You were the chosen one! It was said you would destroy the Sith, not join them! Bring balance to the Force, not leave it in darkness!Anakin: I HATE YOU!Obi-Wan: ...you were my brother, Anakin... I loved you.
- Notice Obi-Wan's expression when he returns to Padmé's star skiff. He just sighs, and sits there in horrified silence.
- We get to see Darth Vader's first chronological appearance in his iconic armor, and the great price at which it came.
Darth Vader: Where is Padmé? Is she safe? Is she all right?Darth Sidious: It seems, in your anger... you killed her.
- Think about it: Vader is in agony. All of his remaining limbs have been hacked off, his entire body is burned, his lungs have been destroyed by Mustafar's hot fumes, his eyes are dried out, his eardrums have partially melted, and he's just had a cybernetic reconstruction without anesthetic. His life-support is noisy, his armor snags and pulls on his tender flesh, and his new limbs are crude and clunky. But as long as Padmé is alive, he'll endure the pain. Right?
- Padmé's funeral is completely silent, with the Naboo and the Gungans gathered together to honor her memory. Everyone who cared about her, including her parents, the current Queen Apailana, Boss Nass, Jar Jar Binks and her handmaidens, feel their own kind of grief.
- There's a close-up of her hands, holding the necklace Anakin gave her—to "bring good fortune."
- Notice how, when Obi-Wan delivers baby Luke to Owen and Beru, the former refuses to talk to or acknowledge Obi-Wan. He wanted to get to know Anakin, and feels robbed of a brother, blaming Obi-Wan and the Jedi for his "death".
- After two grueling hours, even with Palpatine's plan fulfilled and a galaxy cast into darkness, we leave with Beru and Owen clutching baby Luke as they look out into Tatooine's sunrise, where the galaxy's new hope will stand nineteen years later.
- Obi-Wan gets a few more words in before leaving Anakin in Mustafar:
"I loved you... But I couldn't save you."
- It's no wonder he believed Luke had no choice but to kill Vader.
- As stated in the last passage on the Quotes page, Vader also tries to kill Palpatine with his Force scream—but the movie's events left him so weak that he can't even do that.