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Tear Jerker: Pokémon: The First Movie
If you ever seen the short, then it's absolutely gut-wrenching for Mewtwo to lose his only human companion (who was a clone of Dr. Fuji's daughter), what makes this tragic is that he lost his memories of her right after she dies.
After Dr. Fuji's stupid moment when he tells Mewtwo he's an experiment, Mewtwo destroyed the lab in his very pissed off moment. Not only does it appear that he's killed all of the scientists in the lab, but he also destroyed what's left of Amber/Ai (but after what he said, at least he's with his daughter now).
Even Mewtwo, of all characters, is affected by it; after spending his whole life believing that Humans Are the Real Monsters, seeing one trying to stop the fight is enough to bring some sanity into him.
The music score "Tears of Life" during the scene is what really hits it home.
Pikachu doesn't start crying until he realizes that even though he's a Magical Defibrilator, his Thundershocks do absolutely nothing about petrification. He still kept shocking Ash, desperately trying to revive him, even though his Thundershocks were doing nothing.
Pikachu just standing there taking hits from his clone refusing to fight is pretty heartwrenching too.
Despite the slight Narm of the scene, (and the song "Brother My Brother") watching the two groups of pokémon practically killing each other to prove which deserved to live was way different from watching a normal match between pokémon. The whole thing was pretty brutal.