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Tear Jerker: Obscurus Lupa
In her 100th episode review of Evil Dead 2, several demon-possessed TGWTG contributors appear and tell her how much she and her videos suck. Though Lupa did put that together herself, it was still really hard to watch.
Her Cameo in Kyle's 50th Review. She was asked to come up with anything, and improved herself telling Kyle how much he sucks. Considering this starts Kyle's Despair Event Horizon (Various other cameos and the movie itself don't help), but considering how they were at least scripted... Jesus, Lupa.
In the post Lupa talks about the effects of living with Never Live It Down, which show the very sad side of this trope as Lupa reveals how she feels about the criticism she's received since June 2012. Each of the female TGWTG contributors have gone through their respective hazings, but Lupa's trial by fire was harsher by far.
Another blow struck her in 2013 when her cat Checkers died at the age of 13. There was a huge outpouring of grief from both TGWTG fans and producers, who all remembered Checkers with fondness and offered support to Allison at this time.