This is based on opinion. Please don't list it on a work's trope example list.
Tear Jerker / Monsters, Inc.
When Sulley says goodbye to Boo. Sulley (at the time) knows he'll never see her again, but Boo's too young and innocent to truly realize what's going on. Once Sulley gives her one last hug and leaves, she runs up to the door and is ready to burst out to give him another 'scare' but all she sees is an empty closet.
Even Roz sounds like she doesn't want to have to do this.
A "tears of happiness" example: the ending. "Boo?" "Kitty!"
When Sulley accidentally scares Boo. The look on her little face is so heartbreaking... like her world just fell apart. And Sulley'sreaction, as he realizes through Boo's trauma just how he's been appearing to so many children in the past, and just what a terrible thing it is to be "Top Scarer".
It becomes even worse after you watch Monsters University and you recall what Dean Hardscrabble told him about his scaring.
Just seeing how much the monsters traumatize children, how corrupt Monsters Inc. really is, and how the main characters in the prequel are unaware of that and strive to be a part of said company makes Monsters University a thoroughly depressing watch if you think about it for too long.
The sequence where Sulley thinks that Boo is being horribly killed by the waste disposal. Appropriately, it's a Shout-Out to the Warner Brothers short "Feed the Kitty", where a dog finds a kitten, hides it from his mistress, and goes through the same torment as it appears his mistress is accidentally baking the poor little thing in a batch of cookies. And audiences then lost it too. Both Boo and the kitten were okay.
Mike: Sulley, what about everything we worked for? Does that matter, huh! And what about Celia? I am never...never going to see her again. Doesn't that matter? What about me? I'm your pal. I'm...I'm your best friend. Don't I matter?
Sulley: I'm sorry, Mike. I'm sorry we're stuck out here. I didn't mean for this to happen, but Boo's in trouble. I think there might be a way to save her if we can just get down to that—
Mike: We? Whoa-whoa-whoa, we? There's no "we" this time, pal. If-if-if you want to go out there and freeze to death, you be my guest. Because you're on your own.
This becomes even more painful if you've seen Monsters University and how hard and long it actually took for them to reach their dream of becoming scarers. Especially with the teasing and put downs by both students and teachers Mike had to suffer with in college because he wasn't good enough to be a scarer no matter how hard he worked at it. To Mike, Sulley was back to being that jerk back in college that cared about no one but himself.
Depending on your point of view Waternoose is this if you see him as a Jerkass Woobie. He was only driven to villainy out of desperation to keep Monsters, Inc. afloat in the midst of the energy crisis. It can be quite heartbreaking seeing him carried away by the CDA accusing Sullivan to be the cause of the energy crisis and that the Monsters Inc will be closed forever thanks to him.
Waternoose: I hope you're happy, Sullivan! Monsters, Inc. is dead! Where is everybody gonna get their scream now!? The energy criris will only get worse because of you!
The worse thing is that when Sulley realises that laughter is more powerful than scream and manages to both save Monsters, Inc and avert the energy crisis, Waternoose does get what he wanted, but unfortunately, he can't share in that victory.
Sulley's Big "NO!" as he slams the door over and over again in anguish when he and Mike are banished.
Imagine how Boo's parents must have been feeling the entire time Boo was gone. They could have spent the entire movie frantically searching for their lost daughter without a clue on where she is.