At the beginning of Far Lands or Bust Episode #178, there's a noticeable emptiness when there is no *WOOF* to begin the episode, which had been tradition for quite some time before Wolfie disappeared during the F Lo B-athon Livestream.
When Wolfie disappears in the 2012 F Lo B-aton, Bdubs makes a comparison to Jurassic Bark. Within 30 seconds, there isn't a dry eye in the house.
In an early 2014 F Lo B episode, Kurt reveals that Speedy; his pet box turtle of nearly 23 years had passed away. He recounts taking her to the pet cemetery and the long drive back his now empty home. By the end he starts to get choked up and takes a moment of silence to compose himself.