The Mellifer's state after Nick had to Save the Villain. It is made even worse because she doesn't understand what Nick is doing.
Lena being captured and turning old. Charlotte and her daughter lived and will live the same thing, unless something is done.
Possibly even more tragic is the extremes they're forced to undertake to avoid that fate. You can see how much Lena hates what she's doing, but she's biologically compelled to it.
Angelina's reaction to her brother Hap's death. This is what solidifies her as an Ensemble Darkhorse.
Rosalee's reaction to her brother's assault. She is so resilient, but you can still see that she is on the verge of breaking down.
The mouse serial killer. The whole episode is basically you feeling horrified, but wishing for him to have a happy ending. Everything is just so close from getting better, even though everyone knows that it won't.
When Martin bursts into tears after being cornered by Nick, saying that his abusive dad is everywhere. Even though he killed several people including said dad, attacked a man in a restaurant, and kidnapped his friend, you can't help but want to give him a big hug.
The bird lady victim of Domestic Abuse. She is just so shy, so fragile and so resigned. She doesn't even want money.
Adalind's home and the scene in it, with her mom. The way Renard treats her after she becomes human.
Nick and Juliette's meeting at the trailer. It becomes clear that Juliette just won't try to understand, because she won't ever trust him.
Juliette and Nick's deteriorating relationship.
Angelina and Monroe's reunion and the sacrifices she makes for him.
The terrified parents and the kidnapped children in la Llorona, but, also, the Llorona herself. "Mis hijos !"
Rosalee having to sit through dinner with her estranged mother and sister. Her mother isn't that bad, but her sister DeEtta keeps on pushing Rosalee's buttons—blaming her for having to move in with their mother after their father died, and going on and on about how Rosalee was too busy partying up the coast and doing drugs to bother coming to the funeral. Rosalee finally snaps and tells them that she was in jail and had lost everything, and by the time she got out and found out about her father's death the rest of the family had stopped talking to her. No wonder she storms out in tears after.
Apparently Rosalee just can't catch a break right now. Monroe proposes, which should just be the happiest thing ever (and it is for like a minute) but then we meet his parents, and it turns out they're horrible bigots that believe in species purity and get on their case about being in a mixed relationship. She walks out in tears with that one too. If that wasn't bad enough, Monroe decides that he's willing to completely sever his relationship with his parents because of Rosalee. Even though it's the most painful thing he's ever done and he's about two seconds away from tears.
Happily, the two end up getting married, and that should be the end of it, right? Nope. The two are faced with vandalism (including a burning brand in their front lawn), and poor Rosalie has the horrific incident of finding a dead fox on her doorstep, with a phone call warning her that unless she leaves Monroe, it'll be her blood next.
He's eventually let into the loop, and promptly has a Heroic BSOD, getting drunk, starting a fight and then getting thrown in jail. Bonus points for the background music being Delta Spirit's "From Now On", which includes the lyrics "I had a vision about this very thing. But it's probably in my head."
No matter how one feels about Adalind, her reaction to her baby being taken from her is brutally hard to watch.
Trubel tells Nick that she's leaving with Joshua to help him take his life back. Nick accepts it and gives them Aunt Marie's old truck. The whole scene has a very "leaving the nest" feel to it.
The fate of the Chupacabra. He is actually a coyotyl infected with a disease that turns him into a bloodthirsty (literally) killer. There's a cure, but he has infected his wife inadvertently, and Rosalee only has enough ingredients in her shop for one dose. The husband grabs the syringe and plunges it into his wife's back, ensuring her survival and his ultimate demise. Her grief-stricken Big NO is heartbreaking.