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Tear Jerker: Grimm
Aunt Marie's death is one for this troper.
The Mellifler's state after Nick had to Save the Villain. It is made even worse because she doesn't understand what Nick is doing.
Lena being captured and turning old. Charlotte and her daughter lived and will live the same thing, unless something is done.
possibly even more tragic is the extremes their forced to undertake to avoid that fate. you can see how much Lena hates what she's doing, but she's biologically compelled to it.
Angelina's reaction to her brother Hap's death. This is what solidifies her as an Ensemble Darkhorse.
Rosalee's reaction to her brother's assault. She is so resilient, but you can still see that she is on the verge of breaking down.
The mouse serial killer. The whole episode is basically you feeling horrified, but wishing for him to have a happy ending. Everything is just so close from getting better, even though everyone knows that it won't.
The bird lady victim of Domestic Abuse. She is just so shy, so fragile and so resigned. She doesn't even want money.
Adalind's home and the scene in it, with her mom. The way Renard treats her after she becomes human.
Nick and Juliette's meeting at the trailer. It becomes clear that Juliette just won't try to understand, because she won't ever trust him.
Juliette and Nick's deteriorating relationship.
Angelina and Monroe's reunion and the sacrifices she makes for him.
The terrified parents and the kidnapped children in la Llorona, but, also, the Llorona herself. "Mis hijos !"