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Mid-Season Twist: April begins her training with Splinter and the Turtles acquire and actually manage to keep a live canister of Ooze.
Red Eyes, Take Warning: Falco's eyes become bloodshot after he injects himself with the psychic neuro-chemical.
Replacement Goldfish: Played pretty straight when Splinter asks April to train as a ninja because of her gift of empathy. Especially notable because afterwards it's obvious he's thinking of his lost daughter, Miwa.
Would Not Hit a Girl: Played with. The monkey beats the tar out of Donnie and starts to go after April. He gets as far as grabbing her and roaring in her face, but backs off after she talks to him. It's later revealed that the monkey was reading their emotions, so he was hostile when Donnie was hostile and calm when April was calm. Her gender had nothing to do with it.