When Shredder plans to create an army of mutants, the Turtles must rely on the help of the Pulverizer.
- Absentee Actor: Once again April is nowhere to be seen, but special mention goes to this episode as April was told she couldn't leave the lair in the previous episode. Though to be fair she still has to get some stuff from topside.
- Aesop Amnesia: The Turtles once again forget to not have an ego about how good they are in the beginning of the episode. Hence why Splinter decided to step in before it bites the turtle in the ass.
- Averted with Donatello's moments with Pulverizer since he remembers Splinter's words about how he's responsible for what happens to Pulverizer since he's the one who trained him.
- Be Careful What You Wish For: Pulverizer wanted desperately to become a mutant and as the Turtles explicitly point out in the episode, he got his wish.
- Bilingual Bonus: The Foot motorcycle sashimono reveal The Shredder's family name is written as 小禄note .
- Blob Monster: What happens to Pulverizer when he gets mutated. Also overlaps with Body Horror, as all of his organs are clearly visible inside his goo-body.
- Call Back: Where does the Pulverizer go to continue his ninja training? A Chris Bradford dojo.
- Cannon Fodder: This is Pulverizer's job in the Foot, they gave him the rank ashigaru sha.
- Cerebus Syndrome: Done in a single episode no less. It starts out as a rather whacky episode since the Pulverizer is in it... and then he mutates and this episode arguable becomes the DARKEST episode in the series so far.
- Cool Bike: Now the entire Foot Clan has them, black chopper-style hogs with an Eastern dragon motif as the front half of the bike. Seeing Fishface driving one is pretty surreal.
- Curb-Stomp Battle: The Turtles doing this to the Foot so often is what drives much of this episode; both Splinter's warning about complacency and Shredder's need for a force able to match the Turtles. See Lowered Recruiting Standards below.
- Downer Ending: Timothy has become Mutagen Man and the villains get away, with the Shredder and the sole Kraang thinking of a team up.
- Even Evil Has Standards: The Kraang under Shredder's captivity seems slightly disturbed by Shredder's abuse and threats towards Dogpound.
- Foreshadowing: The comic book Michelangelo holds up has 80's Mutagen Man (with the line "Here comes LIVING X-RAY!") on the cover.
- Funny Background Event: When Leonardo is criticizing Raphael and Michelangelo on how life isn't a game, Donatello can be seen and heard in the background saying "Hey, coins!" and ducking to pick them up, with a Mario coin sound effect being heard.
- Gone Horribly Right: Pulverizer wanted to be a mutant. Now he is one.
- Gory Discretion Shot: Averted. Unlike other characters whose mutations were shadowed or off-screen, Pulverizer's is in full view and enjoys several close-ups.
- His Name Really Is Barkeep: Pulverizer tries to pull this trope, but it's revealed that his name is Timothy.
- Hollywood Acid: Pulverizer get this after his transformation to Mutagen Man.
- Hypocritical Humor:
Fishface (to the Turtles): I'll deal with you soon enough, freaks!
- Improvised Weapon User: Splinter tries to teach the Turtles to be this. It doesn't work until the battle in the warehouse.
- Lowered Recruiting Standards: Dogpound has started doing this to bolster the numbers of the Foot. The Turtles are disappointed in the recent decline of enemy competence.
Michelangelo: (disappointed) Dude, it's like we just cleared a video game on "easy".
Leonardo: The Foot soldiers haven't been much of a challenge lately. Wonder why.
- Even Shredder is disappointed:
Shredder: The ones left are hardly worthy of wearing the emblem of the Foot.
- Later confirmed to the Turtles when the Pulverizer explains how he joined the Foot Clan:
Michelangelo: Wow, they must really be hurting for dudes.
- Never My Fault: Doesn't actually come up verbally, but there are shades of this when Pulverizer starts attacking the Turtles after his mutation.
- Or he's just feral now it's unknown
- Oh, Crap: The Kraang pulled this when he realized what Shredder was going to use the mutagen for.
- Painful Transformation: "It burns!"
- Tempting Fate: Raphael asks Xever "What'chya gonna do now?" after hitting him with the trash cannon. Xever responds by taking out their back wheel. It is then lampshaded with him asking why he asks stuff like that.
- Too Dumb to Live: Pulverizer, big time. Hell Donnie tries to save him multiple times from dosing himself with the highly dangerous and completely unpredictable mutagen and even mentions how dangerous the gunk is, yet Timothy still wants to take it! Eventually, he succeeds.
- Villain Team-Up: The ending shows that Shredder and the Kraang he kidnapped are considering working together to defeat the turtles.
- What the Fu Are You Doing?: When the Turtles try to fight with each others' weapons, Hilarity Ensues. And Pulverizer, naturally.