Movie:
In Texas, fugitive bank robber brothers Seth and Richter Gecko hold up a liquor store, forcing the clerk Pete Bottoms to give them money. In a shootout, they kill Pete and Ranger Earl McGraw when the former calls the latter for help. They inadvertently destroy the building as they leave.Planning to move to Mexico, the Geckos go to hide out in a motel with a bank clerk they had previously taken hostage. When they watch in the news about how they're wanted, Seth goes to get food, only to find that Richie has raped and murdered the clerk upon returning, much to his anger. However, he promises him that everything will be fine when they get to Mexico.
Jacob Fuller, a pastor experiencing a crisis of faith brought on by the death of his wife, is on vacation with his teenage children Scott and Kate in their RV. They stop at the motel and get kidnapped by the Geckos, who force the Fullers to smuggle them over the US-Mexico border.
In Mexico, they arrive at the Titty Twister, a saloon-esque strip club in the desert, where the Geckos will be met by their contact, Carlos, at dawn. Carlos will escort them to sanctuary at "El Rey", a place of safety for fugitives from justice whose admission fee is 30% of everything they have. When Richie complains to Seth that the fee is too high, Seth tells him it's non-negotiable. At the suggestion of Jacob upon winning a semantics argument, they decide to enter the club.
In the Titty Twister, people are doing things as usual in how a strip club would be like, such as watching the strippes, drinking, playing tabletop games and enjoying a band concert. When a fight starts, the employees reveal themselves as vampires and kill most of the patrons.
Richie is bitten by the lead stripper, Santanico Pandemonium, and dies but Seth manages to kill her by shooting at a chandelier above her that impales her. Only Seth, Jacob, Kate, Scott, a biker named Sex Machine and Vietnam veteran Frost survive the wave. The rest of the people are reborn as vampires, including Richie, forcing the survivors to kill them all in a second wave. When an army of vampires, in bat form, assembles outside, the survivors lock themselves inside but Sex Machine is bitten, becomes a vampire and bites Frost and Jacob. Frost throws Sex Machine through the door, allowing the vampires to enter while Frost turns into one of them.
Seth, Kate and Scott escape to a storeroom, followed shortly by an injured but still alive Jacob, brandishing a shotgun. In the storeroom, they fashion weapons from truck cargo the vampires have looted from past victims, including a stake mounted on a pneumatic drill, a crossbow and holy water, which requires Jacob to recover his faith to bless. Jacob, knowing he will soon become a vampire, makes Scott and Kate promise to kill him when he changes.
The group makes their final assault on the undead. Sex Machine revives, mutating into a large rat-like creature; he tries to attack Seth but is killed. When Jacob becomes a vampire, Scott hesitates to kill him, allowing Jacob to bite him. Scott hits Jacob with holy water and shoots him. Scott is overwhelmed by vampires, who begin to devour him; he begs for death and Kate shoots him. As vampires surround Kate and Seth, streams of morning light enter through bullet holes in the building, making the vampires back away. Carlos arrives and his bodyguards blast the door open, letting in the sunlight and killing the vampires.
Seth chastises Carlos for his poor choice of meeting place and negotiates a smaller tribute for his admission to El Rey. Kate asks Seth if she can go with him to El Rey, but he refuses, apparently concerned for her safety, leaving her with some cash. Kate drives away in the RV, leaving the Titty Twister — revealed to be the top of a partially buried Aztec temple — behind.
