Late at night, Selina Kyle visits a tiger exhibit at the Gotham Zoo, telling the tiger that she finds it cruel to keep it in captivity. Suddenly, an apelike man shoots her with a tranquilizer dart and carries her off, knocking a guard into the tiger's enclosure.Bruce Wayne waits to have dinner with Selina, and learns that she took a detour at the zoo. When he arrives, the rescued guard is recounting to the police what happened to Selina. Bruce notices the tranquilizer dart.Selina awakens in a lab where a humanoid cat-creature approaches her. A man called Dr. Emile Dorian introduces the creature as Tygrus and notes that Tygrus likes her. Dr. Dorian explains that he is a geneticist who creates various animal and human hybrids such as Garth, the ape-man who kidnapped her and serves Dorian, and Tygrus is the pinnacle of his art. Dr. Dorian is very fond of cats, hence why he chose Selina for his next experiment; he injects her with a serum.Batman consults Dr. Kirk Langstrom about the compound in the dart. Langstrom identifies the compound as T-99, a compound used in splicing created by Dorian, who Langstrom used to work with. Langstrom explains that Dorian combined cat DNA with other species but people protested his gruesome studies, so he retired to an island off the coast to continue his work. Batman flies the Batwing to the island where Dr. Dorian reveals Selina as a literal Catwoman.Batman is horrified and demands Dorian fix her but Dorian thinks he did Selina a favor. Tygrus appears and looks lovingly at Selina, but she just glares at him. Seeing Batman as a rival, Tygrus attacks him as Dorian likens Tygrus to William Blake's poem. Selina intervenes and Dorian notes that her human side is still more dominant. He plans to give her another injection and use Batman to test Tygrus's skills.Batman fights Tygrus and discovers that he can talk. Tygrus says Dr. Dorian, his "father", told him that with Batman gone, Selina would love him. Batman tells him not to count on it. Selina escapes Dorian's clutches and tells Tygrus that Batman is not the enemy and killing him will not win her affections. Tygrus, having learned the opposite from his father, runs off torn and confused.An angry Dr. Dorian scolds Tygrus for not killing Batman. When Batman and Selina show up, Dorian blames Selina for ruining Tygrus and tries to shoot her. Tygrus stops Dorian, who then threatens to kill him. Enraged that his father turned on him, Tygrus goes on a rampage, destroying the lab.Selina and Batman escape with an unconscious Garth. Tygrus emerges from the ruins carrying the unconscious Dr. Dorian for Batman to take to the authorities. Tygrus invites Selina to stay with him on the island, but she says she needs to be human again. Tygrus gives her the antidote and walks off as it starts to rain. Selina says, "Wait! Won't you come with us? There's nothing for you here." Tygrus solemnly replies, "There's nothing for me anywhere."
Batman: (voice over)—-
Tyger, Tyger, burning bright,
In the forests of the night
What immortal hand or eye,
Could frame thy fearful symmetry?