Brick Joke: GOB mistakes his Christian girlfriend (Ann Veal) asking him to embrace "the Holy Trinity" as asking to have a threesome. Near the end of the episode, Girl Michael thought Lindsay and Tobias wanted to have a "Holy Trinity" with him.
Cluster Bleep Bomb: When Nellie is laying down her ground rules in the Bluth company office.
Callback: There are callbacks to "Mr. F" (when Lindsay reminds Michael of the incident, and when Michael realises that Franklin is "Frank"), and to George Sr's cheating.
Continuity Nod: GOB lying about his sex life, which was a major issue when he tried to divorce his wife, but refused to admit that he never consummated the marriage.
Dude, She's Like, in a Coma!: Buster's nurse, Nurse Julia Adelaide, falls in love with him while he fakes being in a coma, and eventually crawls into bed with him. Apparently, she exclusively seeks out this trope, as she has had this exact situation with various other patients before. At the end of the episode, John Van Heusen, the representative trying to keep Buster alive, crawls in with Buster too, much to his horror.
Platonic Prostitution: In "Family Ties," Nellie's clients never seem to end up having sex with her, and end up crying on the floor about their emotional issues. In a flashback, George sobs about his inability to make Oscar jealous with his money; GOB sobs about his inability to get Michael's approval; and all of the employees of the Bluth company mostly ended up doing this too when she was hired accidentally as an in-house prostitute. This doesn't stop both GOB and at least one of the employees from pretending they actually did have sex with her.