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It's very much a blink-and-miss-it moment but when Soundwave is busy taking over the observatory, he is shown going past a human soldier that is shown slumped against a wall, almost certainly dead by his hand.
Fowler holds a Mook over the side of a speeding Optimus. High speed chases and driving besides tree branches don't mix, kids. Throughout the rest of the episode, multiple casualties amount, but this one stands out.
Knock Out: (In response to Starscream's order to cease street racing) Buff this.
Starscream cornering Miko in the cave has some outright pedophilic undertones:
Starscream: You like playing with the big robots, don't you, little girl?
One Shall Rise, Part 1
Agent Fowler storms into the Autobot base and says:
Agent Fowler: What have you learned, Prime? And you'd better not blow smoke up my-(notices June standing nearby) Er, oh, ahaha, Mrs. Darby, what a nice surprise!
Operation:Bumblebee, Part 2
Knock Out, having recently obtained a Cybertronian artifact, asks Megatron what it is. Megatron demonstrates that it's a shield generator by firing at him with no prior warning. After it narrowly works, Knock Out gives us the following line:
The bearings joke again, this time from Wheeljack.
Wheeljack:(To Vehicons) Where is Screamie? Doesn't have the bearings to do this himself?
Chain of Command
Apparently "bearings" isn't the only stand-in for "balls," as Wheeljack demonstrates when he remembers that he's carrying a passenger (possibly an unintentional example, but just as easily not).
Wheeljack: "Ah, bolts."
Knock Out rather openly complains that the idea of humans "interfacing" disgusts him.
Knock Out mentions "a pair of Autobots riding [his] rear" in his status update.
This example comes from the otherwise tame Hungarian dub. Starscream's line "Curse you and your logic!" is translated roughly as "Go screw your logic!" — although the swear word he used ("cseszd meg") is a tad more vulgar than "screw".