Beatrice: Are you here to make fun of me too?
Plastic Man: Was that a joke?
Batman: From a ruthless, obsessive creature of the night? Don't be absurd.
— Formerly Known as the Justice League
"This isn't funny, Dean! The voice says I'm almost out of minutes."
— Castiel (talking on a cell phone and standing on a street corner), Supernatural
Dean: Cas, I'm human, I need sleep. Just give me, like, four hours, man.
Castiel: ...Four hours? (Dean hangs up) I'll just... wait here, then. (Stands perfectly still, staring off into space)
Garak: Mister Worf, you're no fun at all.
"I am not interested in having fun."
— Tuvok, Star Trek: Voyager
"EDI has a great sense of humor. Or at least a really funny lack of one."
— Kasumi Goto, Mass Effect 2
Jade: (to Billie) Okay, okay, sometimes you're no fun at all.
Ayla: (walks in) And speaking of no fun at all, I'm here too.
Billie: But I'm more fun than Ayla, right?
Jade: Oh yeah, everyone's more fun than Ayla.
Ayla: In fact, I'm changing my name to... No Fun Guy!
Billie: Hey Jade, that could be like your arch-enemy.
Ayla: That's right... Giggle Girl! Once I unleash my latest invention, the Federal Tax Code Emulsifier, no one will be having fun ever again!
Jade: Giggle Girl doesn't know the meaning of the word defeat!
Ayla: And she doesn't know the meaning of the word 'rationality' either!
Billie: Or the meaning of the word 'crayon'!
Jade: Giggle Girl does so know the meaning of the word crayon! It's the cute little pink thing I use to write 'No Fun Guy is a big poopyhead' all over your secret lair.
Ayla: D'oh! I knew I shouldn't have shot all those henchmen even after they said they didn't do it...
Jade: Hah! Giggle Girl always defeats the forces of boredom and no-fun-ness!
Ayla: But can she defeat...your next final exam?
Jade: Noooo! Not that!
Billie: (announcer voice) Has No Fun Guy defeated our heroine? Can she find a way out of this hideous trap? Tune in next week for the further adventures of GIGGLE GIRL!
Lord Downey: Is this a joke?
Krusty the Klown: Free comedy tip, Slick: the pie gag's only funny when the sap's got dignity.
(Sees Sideshow Bob)
Krusty the Klown: Like that guy! Hey Hal, pie-job for Lord Autumnbottom there!
Jack: Hey, Optimus, wanna see something funny?
Angel: I'm brooding.
Lorne: You're watching hockey!
Angel: Yeah, but my team is losing.
Angel: I do not have puppet cancer!
— Angel, "Life of the Party" and "Smile Time"
It's hilarious how you have no sense of humor!
— Hades to Dark Pit, Kid Icarus: Uprising
We take it seriously; some of us take it quite seriously. That itself is funny.