[[folder:Anime and Manga]]

->'''Saionji:''' You're a girl?\\
'''Utena:''' I don't remember saying I was a boy!
-->-- ''[[Anime/RevolutionaryGirlUtena Adolescence of Utena]]''


[[folder:Comic Books]]

->'''[[IAmNotShazam Kid]] [[VideoGame/KidIcarus Icarus]]:''' Gosh-a-cus, Princess Lana! [[{{Franchise/Metroid}} Samus]] is super-duper-a-cus!\\
'''Lana:''' Well... Samus is a veteran of many impossible missions! Samus is a super-powered cyborg! Samus is the greatest space hunter in the Galactic Federation! (''Samus removes her helmet'') Samus is a... woman?!\\
'''Kevin:''' Whew! You sure are!
-->-- ''WesternAnimation/CaptainNTheGameMaster'' comic story "Money Changes Everything"

->What? Everyone just ''assumed'' I was male. The ruthless, Nietzschean will to power, however, isn't gender-specific.
-->--'''Deathmonger''', ''ComicBook/{{Empowered}}'' vol. 9


[[folder:Fan Fiction]]

->Captain Borghese's wide-eyed stare finally tore away from staring at where the plasma blast had struck. He blinked in surprise to see the smiling woman revealed by the now transparent helmet.
-->-- [[Anime/NeonGenesisEvangelion Misato Katsuragi]] clears her PoweredArmor's faceplate, ''Fanfic/ACrownOfStars'' [[http://forums.sufficientvelocity.com/threads/a-crown-of-stars-nge-ou.11766/page-17#post-2733485 chapter 72]]

->Wasn't expecting me to be a woman, were you?
-->-- '''[[TropeNamer Samus Aran]]''' to [[Franchise/SuperMarioBros Mario]], ''FanFic/PaperMarioX'', Prologue: How to Steal a Castle

->Whoa! Sorry about you suit... ''mam?!'' GUYS! We've been chased by a girl!
-->-- '''Franchise/SonicTheHedgehog''', right after finding out [[TropeNamer Samus]]'s gender, ''FanFic/PaperMarioX'', Chapter 61: The Mixed-Up Paths of Bowser's Castle

->'''Gloomtail''': WHAAAAATTT? You destroyed my precious younger sister!\\
'''[[Franchise/TheLegendOfZelda Link]]''': Hooktail WAS a girl!\\
'''Flurrie''': Wait, you're Hooktail's brother?\\
'''Franchise/{{Kirby}}''': But what if "he" is a girl too...\\
'''Flurrie''': No, I'm sure this guy's a... guy...
-->-- ''FanFic/PaperMarioX 2'', Chapter 72: The Tail of Gloom and Its Doom

->'''[[Franchise/{{Alien}} Pvt. Vasquez]]:''' Damn! (''{{beat}}'') I totally mistook you for a man!\\
'''[[TropeNamer Samus]]:''' Samus is a woman's name.
-->-- ''Xenophobia'', [[https://www.fanfiction.net/s/12137117/9/Xenophobia Chapter 8: Unknown Species, Unknown Origin]]

->'''[[Anime/CrossAnge Ange]]:''' No, how is that...\\
'''Tusk:''' It's a... It's a...\\
'''Momoka:''' '''[[LargeHam IT'S]]''' '''[[ChewingTheScenery A SHE!]]'''\\
'''Ange:''' You're a woman in that armor all along?!\\
'''[[TropeNamer Samus]]:''' Yes. Do you have a problem with that?
-->-- ''Metroid: Kamen Rider Generations Vol. 2 -- Ex-Aid Era feat. Cross Ange'', "[[https://www.fanfiction.net/s/12213237/2/Metroid-Kamen-Rider-Generations-Vol-2-Ex-Aid-Era-feat-Cross-Ange Episode 002: Fallen Angel, part 2]]"


[[folder:Film - Live-Action]]

->'''Tony Stark:''' Good God, you're a woman! I honestly- I couldn't have called that. I mean, I would apologize, but isn't that what we're going for here? I thought of you as a soldier first.\\
'''Ramirez:''' I'm an airman.\\
'''Tony Stark:''' Well, you have actually excellent bone structure there. I'm kinda having a hard time not looking at you now. Is that weird?
-->-- ''Film/IronMan''

->'''Neo:''' I just thought you were a guy.\\
'''Trinity:''' [[ThereAreNoGirlsOnTheInternet Most guys do.]]
-->-- ''Film/TheMatrix''

->'''Sgt. Hondo Harrelson:''' Sorry, wrong room.\\
'''Officer Sanchez:''' Who were you looking for?\\
'''Hondo:''' Chris Sanchez.\\
'''Sanchez:''' I'm Chris Sanchez.\\
'''Hondo:''' ''You're'' Chris Sanchez?
-->-- ''Film/{{SWAT}}''



->No wonder you go by Jack. I have never heard of anyone named Jacqueline who was not a [[ExplainExplainOhCrap giiirl oh crap.]]
-->-- '''Wreth''', ''And Then There Were Monsters''

->The [[[PoweredArmor power-armoured]]] figure halted a couple of metres away and, to my astonishment, addressed me by name.\\
"Hello Ciaphas," it said, though a vox unit on its chest. The voice sounded familiar, although I couldn't be entirely sure until a golden gauntlet rose to push back its visor. It opened, with a hiss of breaking atmospheric seals, and a well-remembered face, framed with golden hair, grinned at me, devilment dancing as always in the depthless blue eyes. "We really must stop meeting like this."\\
Clearly enjoying my stupedfied expression, which by this point I'm bound to admit would have done credit to Jurgen, Amberley's smile widened still further.\\
With all due modesty, I have to say I recovered remarkably fast under the circumstances.[[note]]'''Inquisitor Amberley Vail:''' [[LemonyNarrator So he says. I recall a distinct resemblance to a stuffed fish for quite some time.]][[/note]]
-->-- '''Commissar Ciaphas Cain''', ''[[Literature/CiaphasCain Duty Calls]]''

->'''Kitai:''' Have I ever attempted to deceive you?\\
'''Tavi:''' The first night I met you, you gave me that [scar] with one of those stone knives. And I thought you were a boy.\\
'''Kitai:''' You are slow and stupid. We both know this. But have I ever deceived you?
-->-- ''[[Literature/CodexAlera Academ's Fury]]''


[[folder:Live-Action TV]]

->'''Sherlock:''' So who is [[TheVoice the man I spoke to on the phone?]] [[TheGhost The man who said he was Moriarty?]]\\
'''Moriarty:''' You talked to one of my Lieutenants. He has over the years played the role repeatedly and with great conviction. More often than not he has done so to protect my identity. Other times it was because I suspected some potential clients might... [[StayInTheKitchen struggle with my gender]]. As if men has a monopoly on murder.
-->-- ''Series/{{Elementary}}''



->Must restore peace! [[CollapsingLair Must escape]]! Peace in the universe is once again restored! HOLYCRAPIMAGIRL!!!
-->-- '''Music/{{Brentalfloss}}''', "''{{VideoGame/Metroid}}'' in 10 Seconds"

->'''[[Franchise/{{Metroid}} Kraid]]''': [[BadassBoast I'm the strongest dragon that you've ever seen]]\\
You're gonna die, [[ThisIsForEmphasisBitch motherfucker]], [[AttackOfThe50FootWhatever I take up five screens!]]\\
[[SwallowedWhole I'm gonna swallow you whole, and then you'll go down easy!]]\\
Then you'll be digested and converted to feces!\\
[[TheUnmasking I'll take off your helmet, see the fear in your eyes]]\\
[[MaleGaze And your beautiful hair, and your well-toned thighs]]\\
[[DistractedByTheSexy And your pillowy lips and...]] [[SamusIsAGirl wait, you're not a guy?]]\\
'''[[TropeNamer Samus]]''': [[BerserkButton Is that a problem, you FUCK?!]]\\
'''Kraid''': Uh, no, {{prepare to die}}!\\
''Kraid was hesitating but Samus was set to fight''\\
''She got into [[BoobsAndButtPose a battle stance that made her butt look super-tight]]''\\
''Then Kraid said''\\
'''Kraid:''' [[VillainousCrush No wait, I think there has been a gigantic mistake]]\\
[[ThroughHisStomach I actually just wanted to give you desserts I have baked!]]\\
''But Samus said''\\
'''Samus:''' What pisses me off most in this world\\
Is when enemies get nice when they all find out I'm a girl!\\
So do me a favor, and take your cakes and your pies,\\
''[[AssShove And shove them so far up your ass that they end up behind your eyes!]]''\\
Stop treating me like I'm a sex object,\\
[[BigBad Mother Brain]]'s a woman and ''she'' gets respect!\\
'''Mother Brain:''' Yeah, Kraid, you never talk to ''me'' that way!\\
'''Kraid:''' [[SarcasmMode Oh, gosh, I wonder if it's because you're]] [[MyBrainIsBig a huge disgusting brain?]]
-->-- '''Music/{{Starbomb}}''', "Regretroid"


[[folder:Video Games]]

->Oh man, don't tell me I've been slaying hot girls this whole time.
-->-- '''Eddie Riggs''', after one of the evil druids he was about to kill turned out to be an ally, ''VideoGame/BrutalLegend''

->'''[[TheHero Chrom]]''': Wait, you're... you're a woman?\\
'''[[spoiler: "Marth"]]''': [[LampshadeHanging And quite the actress, too.]] [[PaperThinDisguise Honestly, I'm surprised you didn't figure it out until just now.]]
-->-- ''VideoGame/FireEmblemAwakening''

->Hold on, the Assassin is a chick? How did I miss ''that?''
-->-- '''The Assault''', ''VideoGame/MondayNightCombat''

->'''Shadow [[spoiler:Naoto]]''': "[[spoiler:Naoto]]"... Such a cool, manly name! But a name doesn't change the truth. It doesn't let you cross the barrier between the sexes. How could you become an ideal man when you were never male to begin with...?\\
'''Yosuke''': Wait, what...? Did he... Did I hear that right!?\\
'''Kanji''': H-He's not a guy!?
-->-- ''VideoGame/Persona4''

->'''Akira:''' Brother, you're alive!\\
'''Daigo:''' I'm sorry, Akira. It must have been tough for a lady like yourself...\\
'''Edge:''' What? His voice... it sounds like...!\\
'''Gan:''' A lady like yourself?!\\
'''Akira:''' I'm sorry I've been deceiving you. I'm not the younger brother of the big boss... I'm his younger sister...
-->-- ''VideoGame/RivalSchools: United By Fate''

->Hey, wouldn't that be kind of cool if it turned out that this person inside the suit was a woman?
-->-- '''Random staff member''' during the development of the original ''VideoGame/{{Metroid}}''. Now we're here.



->'''Luigi''': It's not fair, Link. The only people who flirt with me are other guys.\\
'''Link''': Listen, if this is about [[NoodleIncident that incident in '93]], it was a really awkward time for me and I didn't--\\
'''Luigi''': No. This ''bounty hunter'' guy tried to pick me up at the bar last night. Said he was only ''planetside'' for the evening and wanted to show me the time of my life. Couldn't even see his face under all that ''orange armor''...\\
'''Link''': Wait... Did this "guy" give you his name?\\
'''Luigi''': Yeah. Began with a 'S'. Sam... or something.\\
'''Link''': ''[[TropeNamer Samus]]?''\\
'''Luigi''': ''That's it!'' I told him I didn't swing that way...\\
'''Link''': ''({{facepalm}}ing)'' Oh, by Ganon's ''pork fetish'', Luigi...\\
'''Luigi''': What? What is it?\\
'''Link''': ''Nothing''. Not a ''gosh-darn thing''. ''Say!'' You wanna come over and cry yourself to sleep on my couch for ''no reason whatsoever!?''
-->-- ''Webcomic/ItSucksToBeWeegie'', "[[http://www.interrobangstudios.com/potluck/index.php?strip_id=1084 Flirting with Danger]]"

->"No, Sir."\\
"That's 'No, Ma'am'"\\
"No, M... Wait, you're a woman?"
-->-- ''Webcomic/LegostarGalactica''

->My first great triumph against impossible odds brought me a lot of attention. I think my favorite part of the whole affair was revealing to the waiting dignitaries who I am under my suit... The phrase of the day was '''"He's a whaaa?"'''
-->-- '''[[TropeNamer Samus Aran]]''', ''{{VideoGame/Metroid}}: [[http://bobandgeorge.com/comics/metroid Third Derivative]]''


[[folder:Web Original]]

->'''Tucker:''' What is your problem?! Why do you even care if he's captured, I thought you hated that guy anyway for stealing your girlfriend?\\
'''Church:''' I never said I ''hated'' Tex, just that she was the reason we never got married.\\
'''Caboose:''' ..."She?"\\
'''Grif:''' Aha, I ''knew'' it! Only a chick could give me a headache this big!\\
'''Tex:''' What's the matter, never seen a girl before? How long have you guys been out here?
-->-- ''Machinima/RedVsBlue''

->'''Chuck:''' Are you a guy, or a lady? Because I never heard the name Samus before so I don't know if it's a male or female name, and your voice filter sounds kinda unisex so I have no way to tell.\\
(''Samus stares for a while'')\\
'''Samus:''' Female. If that even matters.\\
'''Chuck:''' Thanks, sorry. Just wanna make sure I don't misgender anyone by accident.
-->-- ''Roleplay/WeAreOurAdventuringAvatars''


[[folder:Western Animation]]

->'''Franchise/{{Batman}}:''' Red Claw is a woman?\\
'''Red Claw:''' Is that a problem?\\
'''Batman:''' [[WouldHitAGirl Not at all. I'm an equal-opportunity crime-fighter.]]
-->-- ''WesternAnimation/BatmanTheAnimatedSeries''