Form up behind me. I'm gonna pop one. If the rest of 'em run, great. If they charge us, we just fly away. *BLAM!* ....Uh-oh. Wings.
— Ebbirnoth, Schlock Mercenary
Doctor Bunnigus: Is... is this part of a grenade?
"Commander, the house Phica shield just went back up."
"It seems to be flickering a bit, we can probably breach, bu-"'
"FULL POWER TO SHIELDS! PILOT! HUG THE GROUND! NOW NOW N-"
"THE AI HAS GONE FERAL! NOBODY SAID ANYTHING ABOUT THE AI BEING FERAL!"
[After realizing Jotaro just pierced through his Stand] "Ahh! Aah! AAAH! AAAAHH!! Ohh, what the fuck?! My only regret is... that I never got to say... 'Wryy'-" [Splatters into a blood fountain]
Dio: [grabbing a boat with his bare hands] Enjoy your stay on the S.S. Plummet, Jotaro!
Jotaro: [in the water, too shocked to even emote] That's a boat... [Dio slams the boat on top of him]
— Jojos Bizarre Adventure Abridged, from the same episode as above.
"You manacled me to my death bed, you pickidilly whore!"
— Putman, Club Dread
"I will never forget the look on their faces. All eight of them. Their faces dropped. All their courage and strength was drained right from their bodies. They had a reputation for breaking up bars, but they knew that instant, they'd made a fatal mistake. This time they walked into the wrong bar."
"I fooled you Ethel, I knew you were all along, I can't believe you fell for that ma stuff, I just wanted to see if I could finally, finally make Ethel Rosenberg sing! I WIN!" (flatlines) "Oh fuck."
— Roy Cohn, Angels In America: Perestroika
"She's going to just blast through the walls?! Oh dear mother of God...!"
"Target in range. Prepare to fire on my command. Hm. Bastard's not even changing course."
[Serenity is followed out of the ion cloud by a whole mess of Reaver ships]
"...target the Reavers. Target the Reavers! Target EVERYONE! SOMEBODY FIRE!"
"Harry... The clock on that nine foot nuclear weapon is ticking."
— Chick, Armageddon
"Uh-oh. It's Mr. Laughs!"
"Look to the skies!"
"It's all coming back! All my memories! Right up until Captain Flint pulled my memory circuit so I could never tell anybody about his Booby Trap!" [BOOM!] "Speaking of which..."
— BEN, Treasure Planet
"It's not often I get to do battle with one of my enemies... in the body of one of their allies. I do so love watching them realize that when they strike me, they will hurt the body of their friend, and not me! Oh yes, that's it... that's the look."
— Karnak, Dominic Deegan'
"How can they be jamming us if they don't know... that we're coming..."
"A Westerner faced with a suicide bomber goes to pieces. Believe me, I have seen this. Just as I have seen people's reactions to other stressful situations: criminals in the electric chair, a person in water confronted by sharks. Oh, to be sure, I love to observe the look of pure horror that crosses a man's face when he realizes that he is, without doubt, going to die..."
"And that is the look I'm talking about."
— Jonathon Killian, Scarecrow
— Rikochet from ˇMucha Lucha!
Mock Cousteau Blah: Now it seems as zough we have found ze bubblefish. We are close now. *shines light on what turns out to be Zeeky H. Bomb* When we shine ze light on it-"
Zeeky: Zeeky boogy doog!
— Hellboy, on anything bad.
White Mage: Did you feel that?
Black Belt: What?
White Mage: A great disturbance in the order. As if millions of voices cried out to say "Oh Shit".
Witch: Stupid Light Warriors must have broken my crystal. I keep asking for Lotto numbers and all I get is "The Destroyer is Manifest".
— 8-Bit Theater, upon Black Mage becoming the leader of Hell.
"I'm sorry, but I cannot divulge information about that customer's secret illegal account." [hangs up]
"Oh, crap. I shouldn't have said he was a customer.
Oh, crap. I shouldn't have said it was a secret.
Oh, crap! I certainly shouldn't have said it was illegal." [sighs] "It's too hot today."
— Cayman Islands banker, The Simpsons 3F12 Bart the Fink.
Crais: I know this ship, and something is out of balance!
Stark: You're imagining things... although, this is strange.
Stark: Docking Bay 2 recently performed an atmosphere replenish, as if it'd been opened... but we landed the pod in Bay 1...
Crais: TALYN, SEAL ALL HATCHES!!!
— Farscape, seconds before a Scarran invades the command deck
"First you say it, then you do it! That's why they call it an accident!"
— Bill Cosby, from Bill Cosby, Himself, explaining why your mother's advice about wearing clean underwear in case you get in a car accident is useless if the accident does happen.
"Damn! We in trouble now!"
Dr. Wily: "I did it! I can hardly believe I did it, but I actually did it! I actually brought Bass back to life!"
Bass: "Huh... Wily?!"
Ghetsis: Granted, the moment when someone loses all hope... I really do love to watch that moment.
Ghetsis: I can't wait to see the look on your face when you've lost all hope!
"I love the look on the DM's face here. This is the classic Dungeon Master Thousand Mile Stare, of a hardened DM taken aback at just how fucked a player is, and realizing how boned the whole campaign's become in an instant."
— Spoony, describing Daniel's priceless reaction during his Mazes and Monsters review
King Dedede: (as he and Escargoon are being chased by angry Waddle Dees) We in trouble! Mah own guards is out to get me!
Escargoon: I've heard of hunger strikes before but this is ridiculous!
— Kirby: Right Back at Ya! Episode 92 (Hunger Struck)
Nightmare: (upon seeing Kirby become Star Rod Kirby) The Star Rod?! AAAAAAAAAAAHHHHH!
— Kirby: Right Back at Ya! Episode 100 (Fright to the Finish)
Amelia: And just HOW are we supposed to stop it?
Zelgadis: If common spells and direct attacks won't work on it...
Lina: ...then you have to use an uncommon spell?
Amelia: Yeah, I guess... AAAHH!!
[Scene cuts to a town square, where Amelia is cranking a siren. Gourry is shouting into a megaphone. Zelgadis is merely standing there with a large sweatdrop.]
Amelia: This is a Dragon Slave Alert!
Gourry: All citizens please evacuate as quickly as possible!
— Slayers NEXT Episode 1
Hazama: P-P-Play tag?! There's no way I can outrun them!
— BlazBlue: Continuum Shift, for when Kokonoe, Taokaka, Jubei, and every single Kaka and cat start chasing after him in a similar game to that of tag in his gag ending.
Oh crap, oh crap, oh crap! Running, running, running!
— Caboose as the Red Army carpet-bombs his tank
Morinth: I used to be a duelist. My favorite part is when you see it in your opponent's eyes. They know you're better, and they're going to die
— Soapy Slick, The Life and Times of Scrooge McDuck
Scar: Ah... my friends.
Shenzi: Frie-heh-heh-hends? I thought he said we were the enemy!
Banzai: Yeah. That's what I heard.
Scar: (gets an expression of horror)
Shenzi & Banzai: Ed?
Ed: (laughs evilly as the hyenas close in on Scar)
Scar: No. L-l-l-l-l-let me explain. No. You don't understand. No! I didn't really mean... No, no! Look, I'm sorry I called you... No! NO!
(the hyenas tear right into Scar as the flames consume them)
Gunnery Sergeant Bardue: Okay Elite League, welcome to a little simulation that I like to call DARK TENNYO!
entire team, realizing just how boned they are: Oh fuck.
— "Ayla and the Great Shoulder angel Conspiracy", Whateley Universe
Sir Kull: Did you see it?
Sir Round: See what?
Sir Kull: THAT...
Sir Round: (as screen zooms in on Shadowfall) OH... MY... G- (interrupted by lightning flash) I heard stories but I never dreamed it could be true!
Sir Kull: It's Sepulchure's flying fortress... on the back of the largest dragon that ever existed!
Sir Round: We are so boned!
Sir Kull: Bad choice of words friend...
Sir Round: This is a grave situation!
Sir Kull: Ugh...
(undead minions burst through the door and begin invading Swordhaven Castle)
— Shadow over Swordhaven cutscene in AdventureQuest Worlds
Motoko: (after being shot at by an invisible mass) Shoot the ceiling out! DO IT!
[helicopter hovering overhead complies, making the shower of glass shards short out the attacker's Invisibility Cloak... and revealing it as a Spider Tank]
Motoko: Aahh, shit...! (over radio) It's a tank! Pull out now!
Pilot: Now what are you gonna do? Argue with it?
Regulatory Agency Clearance Delay...WTF DOES THAT MEAN!!!?? I think I just instantly became diabetic. Anyone near north Seattle. Please come fast. I am going to be needing CPR.
—MyNameIsJeff (accompanied by a picture of him breathing into a paper bag), P-P-P-POWERBOOK! prank
"TACTICAL NUKE, INCOMING!!!"
When they see me, their gaze be all crazy, y'all
—Will Smith, Black Suits Comin' (Nod Ya Head)
— Charlie MacDonald, No More Heroes 2: Desperate Struggle
I'm sitting on a bomb trying to disarm it. This is the feeling you get when the bomb bay doors open.
Oh no, I see
And I lost my head
The thought of all the stupid things I'd said
— Coldplay, Trouble
Greetings, General Oliver! The disappointment you're about to experience delights me!"
Takano: (upon Nail Ripper falling into Satoko's pitfall trap created by her) Waah! A trap? (sees Rika & Satoko about to use their final attack) Aah!
Rika & Satoko: 07th Explosion!
(Rika & Satoko's 07th Explosion attack destroys Nail Ripper and sends Takano flying)
Takano: Sorry, Brother! (afterwards becomes A Twinkle in the Sky)
— Higurashi no Naku Koro ni Kira Episode 2
"Oh, fuck me."
—Tyrion Lannister, Game of Thrones S2E09 "Blackwater"
Cell: I just can't wait to see that look of panic and fear.
Trunks: (reacts in shock)
Cell: That's it! That's the look!
— Dragon Ball Z
Jan: Oh fuck, that's an anti-tank rifle. OH FUCK! THAT'S AN ANTI-TANK RIFLE!
Laura: Uh oh!
Harriette: The big uh oh!
—Family Matters, "Walk on the Wild Side"
"We dreamed of creating the world's strongest Pokémon... and we succeeded."
—Dr. Fuji, Pokémon: The First Movie, right before Mewtwo kills him
Angel Eyes: Oh, I almost forgot. He [Angel Eyes's previous victim] paid me a thousand. I think his idea was that I kill you.
[Baker and Angel Eyes share a laugh]
Angel Eyes: But you know the pity is when I'm paid, I always follow my job through. You know that.
Baker: *looks in horror* NO! ANGEL EYES!
[Angel Eyes kills Baker in ruthless fashion]
Narator: The reader may be interested to note that plyers of deep space are often given to prayer. These simple utterances typically take the form of "Oh (Insert name of deity here)." The reader may also be interested to note that, to a man, our heroes would appear to worship excrement.
"Oh my god... they found me. I don't know how, but they found me. RUN FOR IT, MARTY!"
— Doc Brown, Back to the Future
"No! You'll not have me! My power cannot die!"
— The Horned King, The Black Cauldron, right before the Cauldron devours him
Doctor: "I need some ice."
Idi Amin Dada: "Sure, there is plenty in the fridge. Talk to the chef, I have to talk with this man."
Doctor: "I'll be back." (Goes to the fridge, where there are SEVERED HEADS in the ice)"
Idi Amin Dada: "Doctor! For an African, you are looking very white."
— Rise And Fall Of Idi Amin 
Donut: Hey! Yeah, Simmons! I’m inside the Blue Base! Guess what? Blue Team got a new soldier!
Simmons: *approaches the base* What? They sent another team member? Why would they do that? That doesn't make any sense. *notices the Meta inside the base* OH, FUCK!!! *darts off* Welcometotheneighborhood, seeyoulater!
— Red vs. Blue: Recreation episode 12
— Mami Tomoe from Puella Magi Madoka Magica right before she gets eaten by Charlotte in the manga adaption
"...! How careless of me. I wasn't expecting an audience so soon."
—Iris Zeppelin upon seeing that Grolla has overheard her plans in RosenkreuzStilette Grollschwert.
"When I get my hands on..."
—Mecha-Frieza from Dragon Ball Z, whose threat is interrupted by Trunks slicing him in half after having survived his attack.
"Nonono WAIT! C'mon, give Tao a break! We can go halfsies on the boobies!"
—Taokaka as Platinum prepares to turn her into a Boing Card in her Gag Reel in BlazBlue: Continuum Shift Extend.
Frodo: All right. We put [The One Ring] away. We keep it hidden; we never speak of it again. No one knows it's here, do they? Do they, Gandalf?
Gandalf: There is one other who knew Bilbo had the Ring. I looked everywhere for the creature Gollum, but the enemy found him first. I don't know how long they tortured him, but through the endless screams and inane babble, they discerned two words. (Shire and Baggins).
Frodo: Shire? Baggins? But that would lead them here!
— The Lord of the Rings: The Fellowship Of The Ring
— The Riddler, Batman Forever, after Batman foils his plan to brainwash Gotham.
Discord: What's this? (sees the Elements of Harmony charging up, directly in front of him) No... (Elements are fired directly at his face) NOOOOOOOOOOOOOOOOOOOOOOOO!
"We've created the sun!"
Bender: I can't see. Are we boned?
Leela: Yeah, we're boned.
—Futurama, "Fry and the Slurm Factory"
Dr. Eggman: [After Eggman screws up Sonic's Chaos Control attempt] That... that doesn't look good.
Super Sonic: Oh, for the love of... Eggman, what did you do?! What did you—
—Data, Star Trek: Generations
—Norman Osborn, Spider-Man, right before he gets impaled upon his own glider
"A Sonic Boom?! He's moving faster than the speed of sound!"
—Sam Speed, Sonic X
"Doomsday and Armageddon just had a baby and it is ugly!"
—Sgt. Calhoun, Wreck-It Ralph
"DROP! DROP! DROOOOOP!"
— Sgt. Pinback, Dark Star, when the activated Bomb No. 20 fails to deploy.
"IT'S THE DARK PRECURE!"
"NO! DON'T TOUCH THAT WATER!"
— The Wicked Witch of the West, The Wizard of Oz
"Jeremy's body seemed to know what was coming even before impact, and the rodent part of his brain engaged its natural defenses. He went limp on the way up, hoping the predator would mistake him for dead. Post-impact, it was a different story. While wide awake, each of Jeremy Bullock's limbs tried to escape from his body in different directions, probably to find four different lawyers to sue the fight promoter that allowed this circus. Ike Turner has been in fairer fights than this."
Goblin King: Aaand here he is at last! The hero of our little drama. Just in time for the thrilling climax! But wait— a twist! The "hero" isn't one at all. In fact, he never was. Really, Otto, you're just embarrassing yourself with this charade. Granted, you were never on my level, but being a bad guy suited you. Saving that little lady is your last remaining achievement as Spider-Man. When it all goes boom, you'll have nothing left! Hahahaha!
Peter!Spider-Man: Except the dignity of knowing I never carried a man-purse.
Goblin King: ... it's you.
Peter!Spider-Man: The one and only.