Quotes / Killed Off for Real

Stop all the clocks, cut off the telephone,
Prevent the dog from barking with a juicy bone,
Silence the pianos and with muffled drum
Bring out the coffin, let the mourners come.

"No one stays dead except Bucky, Jason Todd and Uncle Ben."
— An old comic book mantra. Not long after it came into existence, they resurrected Bucky and Jason Todd anyway. Uncle Ben is still dead. Until they brought him back in the Ultimate Universe. Then they killed him again, so no biggie.

Thunderbolt: So, how do I make my shockingly unexpected and triumphant return?
Lightning: You don't.
Thunderbolt: What do you mean, I don't?
Lightning: You don't make a shockingly unexpected and triumphant return. You die. You're dead. You never come back.
Thunderbolt: N-not even for a Reunion Show?
Lightning: Not even for a cameo in the remake.
101 Dalmatians II: Patch's London Adventure (lampshading/parodying the Disney Death trope)

"In any world, real or virtual, once you're dead, you're gone."
Akihiko Kayaba on the 4,000 players who died before Kirito cleared SAO, Sword Art Online, Episode 14, The End of the World

"An empire toppled by its enemies can rise again, but one that crumbles from within, that's dead... forever."
Helmut Zemo, Captain America: Civil War

"There are no continues, my friend."
Revolver Ocelot on if Snake dies in the torture/interrogation segment in Metal Gear Solid

"No resurrections this time."
Thanos after killing Loki, Avengers: Infinity War