In the indie comic Cool Jerk and HomoGal, Cool Jerk tries taglines with mixed results. For example, during a bank robbery: "Who are you?" "The substantial penalty for early withdrawal."
Although the fight between Wolverine and Deadpool in Wolverine Origins has quite a few, there's also a notable aversion when Deadpool notes that the fight is on "for real" and is dead serious when Wolverine starts ditching the one liners and is kicking ass without them.
Tacitly part of Robin's job, to the point that when a recently returned from the dead Jason Todd (ex-Robin II) and the Batman of an alternate universe team up in a fight, the following happens:
Batman: You used to crack jokes at times like these. Jason: Sorry, Batman... Now I just crack heads! Batman: Now that is funny!
Superman: Kara, I think I know how to defeat Warworld — but we'll have to face everything that satellite can throw at us to prove it! Are you game? Supergirl: Are you kidding? Who wants to live forever anyway?
Supergirl: Fine, Skallox! You want to see me get mad? Watch this!
Whenever Popeye uttered the phrase, "That's all I can stands, I can't stands no more!" who ever angered him up to this point was in for a world of hurt.
In The Avengers/New Avengers portion of Original Sin, after Captain America regains his erased memories of the Illuminati and what they have been doing, he confronts Iron Man over it. When Iron Man asks what he was going to do about it, Cap's response?
In The Children's Crusade, Wiccan gets one when Carol Danvers tells him that his team isn't going to win against the Avengers. Right after his one-liner, a massive magical lightning bolt strikes down, knocking all of the Avengers senseless and teleporting his entire team to safety, halfway around the world.