In the indie comic Cool Jerk and HomoGal, Cool Jerk tries taglines with mixed results. For example, during a bank robbery: "Who are you?" "The substantial penalty for early withdrawal."
Although the fight between Wolverine and Deadpool in Wolverine Origins has quite a few, there's also a notable aversion when Deadpool notes that the fight is on "for real" and is dead serious when Wolverine starts ditching the one liners and is kicking ass without them.
Tacitly part of Robin's job, to the point that when a recently returned from the dead Jason Todd (ex-Robin II) and the Batman of an alternate universe team up in a fight, the following happens:
Batman: You used to crack jokes at times like these. Jason: Sorry, Batman... Now I just crack heads! Batman: Now that is funny!
In The Avengers/New Avengers portion of Original Sin, after Captain America regains his erased memories of the Illuminati and what they have been doing, he confronts Iron Man over it. When Iron Man asks what he was going to do about it, Cap's response?