This is based on opinion. Please don't list it on a work's trope example list.
Nightmare Fuel / Toy Story 2
Woody's nightmare in the second film once you realize being dragged into a can of dismembered doll arms is Woody's equivalent of being dragged into a grave by bloody, mutilated human body parts.
The original Toy Story was supposed to have this scene, but instead of being dragged into a trash can of dismembered doll parts he was EATEN ALIVE BY COCKROACHES.
The way Andy acts in the nightmare. The moment he doesn't want Woody anymore, he starts speaking in a creepy monotone and has a glassy, lifeless stare that is truly unnerving. And when he says "Bye Woody" and looks at him one last time before closing the trash can, the lighting and shading of his face make him look almost like something straight out of The Ring. What's worse, certain foreign dubs of the movie distort Andy's voice the moment he drops Woody to make it sound demonic, which coupled with his Uncanny Valley stance makes it look like the poor child is possessed.
Buzz getting disintegrated from the waist up at the beginning. Sure, it's a video game, but yeah.
Jessie's fear of the dark (or at least being put back into storage) is frighteningly realistic. When Woody reveals he doesn't intend to go to Japan with the rest of the gang, Jessie starts freaking out, hyperventilating and stroking her braid. You can tell she's clearly distressed. Later in the movie, Jessie is trapped in the green suitcase and is unable to get out before falling down the compartment slide and loaded onto the plane. By the time Woody reaches her and opens the case, we see Jessie curled up in a fetal position. It's scary how realistic the fear is represented and how close it can hit home.
It's clear that Jessie has some serious psychological problems: claustrophobia from being in storage for so long, separation anxiety from Emily abandoning her, not to mention Pete lying to her for so many years about the fate of their show giving her trust issues. And it's all played very straight. The fact that she's otherwise peppy and excited suggests that she's a Sad Clown.
There's an Easter Egg on the two-disc special edition DVD that shows a rough animation of the scene "Jessie's Song" where Jessie is dropped off for charity. Fair enough. But after the car goes over the hill, it's just silent for a few seconds before the car suddenly careens over the hill and goes all over the place with the tires squealing before it crashes into the boxes. All while in Jessie's point-of-view. Worse is that it's described as a "hilarious outtake". Here's the easter egg.
Stinky Pete finally flipping out at Jessie and Woody when he reveals he's been the one sabotaging Woody's escape plans.
Stinky Pete:FAIR?? I'LL TELL YOU WHAT'S NOT FAIR! SPENDING AN ETERNITY ON A DIME-STORE SHELF WATCHING EVER OTHER TOY BEING SOLD!
Plus the terrifyingly deliberate way he tears Woody's arm in the airport and threatens to dismember him if he doesn't come quietly.
Stinky Pete: Your choice, Woody. You can go to Japan together or in pieces! He fixed you once, he can fix you again!